Mini Samples ELISA Kit for Insulin (INS)

3h

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
    And then add 25uL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.

123.5-10,000pg/mL

48T, 96T, 96T?5, 96T?10, 96T?100

Insulin

Competitive Inhibition

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Insulin (INS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Insulin (INS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Metabolic pathway;Endocrinology;Hormone metabolism;

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

The minimum detectable dose of this kit is typically less than 53.4pg/mL

This assay has high sensitivity and excellent specificity for detection of Mini Samples Insulin (INS).
No significant cross-reactivity or interference between Mini Samples Insulin (INS) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Insulin (INS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Insulin (INS) and unlabeled Mini Samples Insulin (INS) (Standards or samples) with the pre-coated antibody specific to Mini Samples Insulin (INS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Insulin (INS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Insulin (INS) in the sample.

P01326