RevMab Biosciences

anti-His-Tag Rabbit Monoclonal (RM146) (Biotin)

Product Code:
 
REV-31-1048-02
Product Group:
 
Primary Antibodies
Supplier:
 
RevMab Biosciences
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RM146
Regulatory Status:
 
RUO
Target Species:
 
Universal
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
+4°C
1 / 1
Immunocytochemical staining of HeLa cells, using anti-GAPDH RevMAb Clone RM114 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).
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Immunocytochemical staining of HeLa cells, using anti-GAPDH RevMAb Clone RM114 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).

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REV-31-1048-02-C05050 ug£485.00
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This product comes from: Switzerland.
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Further Information

Alternate Names/Synonyms:
Polyhistidin
Concentration:
1 mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.
Handling Advice:
Avoid freeze/thaw cycles.
Immunogen:
Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Labels - Conjugates:
Biotin
Long Description:
Recombinant Antibody. This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells. Applications: WB, IP, ICC, FACS, IHC. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. His-tag is a series of six to nine histidine residues fused to either the carboxyl or amino terminus of a recombinant protein. The small size of the His-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein?s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions allows for the simple purification and detection of His-tagged proteins. His-tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. This His-tag antibody is also available biotin-labeled to meet all your research needs.
NCBI, Uniprot Number:
N/A
Package Type:
Vial
Product Description:
His-tag is a series of six to nine histidine residues fused to either the carboxyl or amino terminus of a recombinant protein. The small size of the His-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein?s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions allows for the simple purification and detection of His-tagged proteins. His-tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. This His-tag antibody is also available biotin-labeled to meet all your research needs.
Purity:
Protein A purified.
Source / Host:
Rabbit
Specificity:
This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibody Conjugates
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.