anti-Phosphothreonine Rabbit Monoclonal (RM102)

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RevMab Biosciences
Product Code: REV-31-1009-00
Product Group: Primary Antibodies
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REV-31-1009-00-C100100 ug£513.00
Quantity:
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Overview

Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM102
Regulatory Status: RUO
Target Species: Universal
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
+4°C

Images

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Immunocytochemistry of serum-starved A431 cells nontreated or treated with Calyculin A/ Okadaic Acid, using RevMAb Clone RM102 at 1/500 dilution (followed by PE conjugated secondary antibody, red) and DAPI (blue).
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Immunocytochemistry of serum-starved A431 cells nontreated or treated with Calyculin A/ Okadaic Acid, using RevMAb Clone RM102 at 1/500 dilution (followed by PE conjugated secondary antibody, red) and DAPI (blue).

Documents

Further Information

Alternate Names/Synonyms:
pThr
Concentration:
1 mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.
Handling Advice:
Avoid freeze/thaw cycles.
Immunogen:
Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide.
Long Description:
Recombinant Antibody. This antibody reacts to threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides. Applications: WB, IP, ICC, IHC, FACS, ELISA . Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. Threonine is an essential amino acid that is used in the biosynthesis of proteins. The threonine residue is susceptible to numerous posttranslational modifications (PTMs). The hydroxyl side-chain can undergo O-linked glycosylation. In addition, threonine residues undergo phosphorylation through the action of a threonine kinase. In its phosphorylated form, it can be referred to as phosphothreonine. Phosphothreonine has three potential coordination sites (carboxyl, amine and phosphate group) and determination of the mode of coordination between phosphorylated ligands and metal ions occurring in an organism is important to explain the function of the phosphothreonine in biological processes.
NCBI, Uniprot Number:
N/A
Package Type:
Vial
Product Description:
Threonine is an essential amino acid that is used in the biosynthesis of proteins. The threonine residue is susceptible to numerous posttranslational modifications (PTMs). The hydroxyl side-chain can undergo O-linked glycosylation. In addition, threonine residues undergo phosphorylation through the action of a threonine kinase. In its phosphorylated form, it can be referred to as phosphothreonine. Phosphothreonine has three potential coordination sites (carboxyl, amine and phosphate group) and determination of the mode of coordination between phosphorylated ligands and metal ions occurring in an organism is important to explain the function of the phosphothreonine in biological processes.
Purity:
Protein A purified.
Source / Host:
Rabbit
Specificity:
This antibody reacts to threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.