Rabbit Fab Anti-Human IgG (H&L) secondary antibody (HRP)
| Code | Size | Price |
|---|
| PAB31783-500ug | 500 ug | £401.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG Fab
Antibody Clonality: Secondary Antibody
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store vial at 4°C prior to restoration.
After reconstitution with 500 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
After reconstitution with 500 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Documents
Further Information
Antigen Target Species:
Human
Conjugate Tag:
HRP
Form:
Lyophilized
Immunogen:
Human IgG whole molecule.
Product Description:
Rabbit Fab polyclonal antibody raised against human IgG (H&L). The antibody is conjugated with Horseradish Peroxidase (HRP).
Recommend Dilutions:
ELISA (1:10000-1:50000)
Immunohistochemistry (1:500-1:2500)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.
Immunohistochemistry (1:500-1:2500)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.
Specificity:
IgG (H&L). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-rabbit serum. No reaction was observed against anti-papain or anti-rabbit IgG F(c).
Storage Buffer:
Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) gentamicin sulfate). Do NOT add sodium azide!