ACE2 Antibody [3F1]

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ProSci
Product Code: PSI-10-604
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-10-604-100ul100ul£729.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: 3F1
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze-thaw cycles.

Images

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<strong>Figure 6 Immunohistochemistry Validation of ACE2 in Mouse Kidney Tissue</strong><br> Paraformaldehyde-fixed and paraffin-embedded mouse kidney tissue incubated with ACE2 antibody [3F1], 10-604, at 1:100, overnight at 4?C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
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<strong>Figure 5 Immunohistochemistry Validation of ACE2 in Human Kidney Tissue</strong><br> Paraformaldehyde-fixed and paraffin-embedded human kidney tissue incubated with ACE2 antibody [3F1], 10-604, at 1:100, overnight at 4?C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
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<strong>Figure 4 Immunohistochemistry Validation of ACE2 in Human Breast Carcinoma Tissue</strong><br> Paraformaldehyde-fixed and paraffin-embedded human breast carcinoma tissue incubated with ACE2 antibody [3F1], 10-604, at 1:100, overnight at 4?C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
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<strong>Figure 3 Immunofluorescence Validation of ACE2 in HepG2 cells</strong><br> IF (ICC) staining with ACE2 antibody [3F1], 10-604, at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
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<strong>Figure 2 Immunofluorescence Validation of ACE2 in MCF-7 cells</strong><br> IF (ICC) staining with ACE2 antibody [3F1], 10-604, at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
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<strong>Figure 1 Western Blot Validation of ACE2</strong><br> Human kidney probed with ACE2 antibody [3F1], 10-604, at 1:1000 overnight at 4?C followed by a conjugated secondary antibody for 60 minutes at 37?C.
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<strong>Figure 6 Immunohistochemistry Validation of ACE2 in Mouse Kidney Tissue</strong><br> Paraformaldehyde-fixed and paraffin-embedded mouse kidney tissue incubated with ACE2 antibody [3F1], 10-604, at 1:100, overnight at 4?C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
<strong>Figure 5 Immunohistochemistry Validation of ACE2 in Human Kidney Tissue</strong><br> Paraformaldehyde-fixed and paraffin-embedded human kidney tissue incubated with ACE2 antibody [3F1], 10-604, at 1:100, overnight at 4?C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
<strong>Figure 4 Immunohistochemistry Validation of ACE2 in Human Breast Carcinoma Tissue</strong><br> Paraformaldehyde-fixed and paraffin-embedded human breast carcinoma tissue incubated with ACE2 antibody [3F1], 10-604, at 1:100, overnight at 4?C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
<strong>Figure 3 Immunofluorescence Validation of ACE2 in HepG2 cells</strong><br> IF (ICC) staining with ACE2 antibody [3F1], 10-604, at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
<strong>Figure 2 Immunofluorescence Validation of ACE2 in MCF-7 cells</strong><br> IF (ICC) staining with ACE2 antibody [3F1], 10-604, at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
<strong>Figure 1 Western Blot Validation of ACE2</strong><br> Human kidney probed with ACE2 antibody [3F1], 10-604, at 1:1000 overnight at 4?C followed by a conjugated secondary antibody for 60 minutes at 37?C.

Documents

Further Information

Additional Names:
Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, Metalloprotease MPROT15, ACEH, ACE2
Application Note:
WB(1:300-1000), IF(ICC)(1:50-200), IHC()
Background:
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
Buffer:
1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
C terminal human Angiotensin Converting Enzyme 2
NCBI Gene ID #:
59272
NCBI Official Name:
Angiotensin-converting enzyme 2
NCBI Official Symbol:
ACE2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q9BYF1
Purification:
Purified by Protein A.
Research Area:
Infectious Disease
Swissprot #:
Q9BYF1
User NOte:
Optimal dilutions for each application to be determined by the researcher.