SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [7E1B]

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ProSci
Product Code: PSI-10-608
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-10-608-100ul100ul£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 7E1B
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze-thaw cycles.

Images

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<strong>Figure 1 Western Blot Validation of SARS-CoV-2 (COVID-19) Nucleocapsid Antibody</strong><br> Lane 1: 50ng, Lane 2: 25ng, Lane 3: 5ng, Lane 4: 2ng , Lane 5: 1ng and Lane 6: 0.5ng of SARS-CoV-2 N protein probed with SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [7E1B], 10-608, at 1:1000 dilution and 4?C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37?C.
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<strong>Figure 1 Western Blot Validation of SARS-CoV-2 (COVID-19) Nucleocapsid Antibody</strong><br> Lane 1: 50ng, Lane 2: 25ng, Lane 3: 5ng, Lane 4: 2ng , Lane 5: 1ng and Lane 6: 0.5ng of SARS-CoV-2 N protein probed with SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [7E1B], 10-608, at 1:1000 dilution and 4?C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37?C.

Documents

Further Information

Additional Names:
SARS-CoV-2 Nucleocapsid Protein, SARS-CoV-2 NP, nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2], novel coronavirus N Protein, novel coronavirus Nucleocapsid Protein, 2019-nCoV Nucleoprotein, 2019-nCoV N, 2019nCoV N, 2019-nCoV N Protein, 2019 peak N Protein, 2019-nCoV nucleocapsid protein.
Application Note:
WB(1:500-2000), ELISA(1:500-1000)
Background:
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Buffer:
0.01M PBS(pH7.4) with 0.1% Proclin300
Concentration:
?1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
NCBI Official Name:
Nucleocapsid phosphoprotein
NCBI Official Symbol:
N
NCBI Organism:
SARS-CoV-2
Physical State:
Liquid
Purification:
Purified by Protein G.
Research Area:
Infectious Disease,COVID-19
User NOte:
Optimal dilutions for each application to be determined by the researcher.