SARS-CoV-2 (COVID-19) Nucleocapsid Antibody

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ProSci
Product Code: PSI-24-031
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-031-100ul100ul£657.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze-thaw cycles.

Images

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<strong>Figure 1 Western Blot Validation</strong><br> Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibody, 24-031, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.
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<strong>Figure 1 Western Blot Validation</strong><br> Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibody, 24-031, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.

Documents

Further Information

Additional Names:
SARS-CoV-2 Nucleocapsid Protein, SARS-CoV-2 NP, nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2], novel coronavirus N Protein, novel coronavirus Nucleocapsid Protein, 2019-nCoV Nucleoprotein, 2019-nCoV N, 2019nCoV N, 2019-nCoV N Protein, 2019 peak N Protein, 2019-nCoV nucleocapsid protein.
Application Note:
WB: 1:1000 - 1:5000, Elisa: 1:1000 - 1:5000, FCM,1:50 - 1:200, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200
Background:
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH 7.3
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein
NCBI Gene ID #:
1489678
NCBI Official Name:
Nucleocapsid phosphoprotein
NCBI Official Symbol:
N
NCBI Organism:
SARS-CoV-2
Physical State:
Liquid
Protein Accession #:
QHD43423.2
Purification:
Affinity purification
Research Area:
Infectious Disease, COVID-19
Swissprot #:
QHD43423.2
User NOte:
Optimal dilutions for each application to be determined by the researcher.