SARS-CoV-2 (COVID-19) Spike Antibody

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ProSci
Product Code: PSI-24-032
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-032-100ul100ul£657.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
Storage:
Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze-thaw cycles.

Images

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<strong>Figure 1 Immunofluorescence Validation of SARS-CoV-2 (COVID-19) Spike in HeLa cells</strong><br> Immunofluorescence analysis of HeLa cells, untreated (right) and SARS-CoV-2 (COVID-19) spike plasmid transfected (left) using SARS-CoV-2 (COVID-19) spike antibody, 24-032, at 1:100 dilution. Blue: DAPI for nuclear staining.
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<strong>Figure 1 Immunofluorescence Validation of SARS-CoV-2 (COVID-19) Spike in HeLa cells</strong><br> Immunofluorescence analysis of HeLa cells, untreated (right) and SARS-CoV-2 (COVID-19) spike plasmid transfected (left) using SARS-CoV-2 (COVID-19) spike antibody, 24-032, at 1:100 dilution. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
Application Note:
Elisa: 1:1000 - 1:5000, FCM,1:50 - 1:200, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200
Background:
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH 7.3
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein
NCBI Gene ID #:
43740568
NCBI Official Name:
Surface glycoprotein
NCBI Official Symbol:
S
NCBI Organism:
SARS-CoV-2
Physical State:
Liquid
Protein Accession #:
QHD43416.1
Purification:
Affinity purification
Research Area:
Infectious Disease, COVID-19
Swissprot #:
QHD43416.1
User NOte:
Optimal dilutions for each application to be determined by the researcher.