ACE2 Antibody

ProSci
Product Code: PSI-24-033
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-033-100ul100ul£686.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze-thaw cycles.

Images

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<strong>Figure 1 Western Blot Validation in Mouse and Rat Tissue Lysates</strong><br>
Loading: 25 ug of lysates per lane.
Antibodies: ACE2 antibody, 24-033, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.
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<strong>Figure 3 Immunohistochemistry Validation of ACE2 in Mouse Kidney Tissue</strong><br> 
Immunohistochemistry of paraffin-embedded mouse kidney tissue using ACE2 antibody, 24-033, at 1:100 dilution (40x lens).

<strong>Figure 1 Western Blot Validation in Mouse and Rat Tissue Lysates</strong><br>
Loading: 25 ug of lysates per lane.
Antibodies: ACE2 antibody, 24-033, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.
<strong>Figure 3 Immunohistochemistry Validation of ACE2 in Mouse Kidney Tissue</strong><br> 
Immunohistochemistry of paraffin-embedded mouse kidney tissue using ACE2 antibody, 24-033, at 1:100 dilution (40x lens).

Further Information

Additional Names:
ACEH, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, SARS-CoV receptor, SARS-CoV-2 receptor
Application Note:
WB: 1:500 - 1:2000, IHC: 1:50 - 1:200
Background:
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH 7.3
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Synthetic Peptide
NCBI Gene ID #:
59272
NCBI Official Name:
Angiotensin-converting enzyme 2
NCBI Official Symbol:
ACE2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q9BYF1
Purification:
Affinity purification
Research Area:
Infectious Disease
Swissprot #:
Q9BYF1
User NOte:
Optimal dilutions for each application to be determined by the researcher.