SARS-CoV-2 (COVID-19) Spike Antibody (cleavage site)

Product Code: PSI-9095

Supplier: ProSci

Code	Size	Price

PSI-9095-0.02mg

0.02mg

£150.00

Quantity:

PSI-9095-0.1mg

0.1mg

£449.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species: Virus

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunofluorescence (IF)
Western Blot (WB)

Storage:

SARS-CoV-2 (COVID-19) Spike antibody (cleavage site) can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 Immunofluorescence Validation of SARS-CoV-2 (COVID-19) Spike (cleavage site) in 293 Transfected Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 spike transfected cells labeling SARS-CoV-2 (COVID-19) Spike (cleavage site) with 9095 at 20 mg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

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<strong>Figure 2 Overexpression Validation in Spike Transfected 293 Cells </strong><br>Loading: 15 μg per lane of 293 cell lysate. Antibodies: SARS-CoV-2 (COVID-19) Spike (cleavage site), 9095 (4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells

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<strong>Figure 3 ELISA Validation with SARS-CoV-2 (COVID-19) Spike Recombinant Protein</strong><br>Antibodies: SARS-CoV-2 (COVID-19) Spike antibody (cleavage site), 9095 (1 μg/mL). A direct ELISA was performed using SARS-CoV-2 (COVID-19) Spike S1 recombinant protein (97-087) as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike antibody (cleavage site) as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 8 ng/mL to 1000ng/mL.

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<strong>Figure 4 Western Blot Validation with SARS-CoV-2 (COVID-19) Spike Recombinant Protein </strong><br>Loading: 50 ng per lane of SARS-CoV-2 (COVID-19) Spike S1 recombinant protein (97-087. Antibodies: SARS-CoV-2 (COVID-19) Spike (cleavage site), 9095, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 2.5 ug/mL and Lane 2: 5 ug/mL.

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Documents

Further Information

Additional Names:

SARS-CoV-2 (COVID-19) Spike Antibody (cleavage site): Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein

Application Note:

WB: 2.5-5 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunofluorescence in human samples. SARS-CoV-2 (COVID-19) Spike Antibody (cleavage site) can be used for the detection of SARS-CoV-2 (COVID-19) Spike protein in ELISA. It will detect 4 ng of free peptide at 1 μg/mL. All other applications and species not yet tested.

Background:

Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a Spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Coronavirus invades cells through Spike (S) glycoproteins, a class I fusion protein. It is the major viral surface protein that coronavirus uses to bind to the human cell surface receptor. It also mediates the fusion of host and viral cell membrane, allowing the virus to enter human cells and begin infection (3). The spike protein is the major target for neutralizing antibodies and vaccine development (4). The protein modeling suggests that there is strong interaction between Spike protein receptor-binding domain and its host receptor angiotensin-converting enzyme 2 (ACE2), which regulate both the cross-species and human-to-human transmissions of COVID-19 (5). The recent study has shown that the SARS-CoV-2 spike protein binds ACE2 with higher affinity than SARS-CoV spike protein (6).

Background References:

Gorbalenya. bioRxiv: 2020.
Hui et al. Int J Infect Dis. 2020;91:264-266.
Belouzard et al. Viruses. 2012;4(6):1011-33.
Lee et al. J Virol. 2006;80(8):4079-87.
Wan et al. J Virol. 2020.
Wrapp et al. Science. 2020.

Buffer:

SARS-CoV-2 (COVID-19) Spike Antibody (cleavage site) is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (15%)

Immunogen:

Anti-SARS-CoV-2 (COVID-19) Spike Antibody (cleavage site) (9095) was raised against a peptide corresponding to 13 amino acids near the center of SARS-CoV-2 (COVID-19) Spike glycoprotein.

The immunogen is located within 650-700 amino acids of SARS-CoV-2 (COVID-19) Spike protein.

ISOFORMS:

SARS-CoV-2 (COVID-19) Spike has one isoform (1273aa).

NCBI Gene ID #:

43740568

NCBI Official Name:

surface glycoprotein

NCBI Official Symbol:

NCBI Organism:

SARS-CoV-2

Physical State:

Liquid

Protein Accession #:

QHD43416

Protein GI Number:

1791269090

Purification:

SARS-CoV-2 (COVID-19) Spike Antibody (cleavage site) is affinity chromatography purified via peptide column.

Research Area:

Infectious Disease,COVID-19

Swissprot #:

P0DTC2

User NOte:

Optimal dilutions for each application to be determined by the researcher.