SARS-CoV-2 (COVID-19) NSP4 Antibody

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ProSci
Product Code: PSI-9175
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9175-0.02mg0.02mg£150.00
Quantity:
PSI-9175-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Virus
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
blue ice or RT
Storage:
SARS-CoV-2 (COVID-19) NSP4 antibody can be stored at 4 ˚ C for three months and -20 ˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

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<strong>Figure 1 ELISA Validation </strong><br>Antibodies: SARS-CoV-2 (COVID-19) NSP4 Antibody, 9175. A direct ELISA was performed using immunogen peptide (9175P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP4 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL
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<strong>Figure 1 ELISA Validation </strong><br>Antibodies: SARS-CoV-2 (COVID-19) NSP4 Antibody, 9175. A direct ELISA was performed using immunogen peptide (9175P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP4 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL

Documents

Further Information

Additional Names:
NSP4
Application Note:
Antibody validated: SARS-CoV-2 (COVID-19) NSP4 antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP4 participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication (3)(4).
Background References:
  • Gorbalenya. bioRxiv: 2020.
  • Hui et al. Int J Infect Dis. 2020;91:264-266.
  • Angelini et. al. DNA Cell Biol. 33:122-127 (2014)
  • Angelini et. al. MBio 4:0-0(2013)
Buffer:
SARS-CoV-2 (COVID-19) NSP4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV NSP4 protein: (85%)
Immunogen:
Anti-SARS-CoV-2 (COVID-19) NSP4 antibody (9173) was raised against a peptide corresponding to 15 amino acids near the center of SARS-CoV-2 (COVID-19) NSP4 protein.

The immunogen is located within 50-100 amino acids of the SARS-CoV-2 (COVID-19) NSP4 protein.
NCBI Gene ID #:
43740578
NCBI Official Name:
ORF1a polyprotein;ORF1ab polyprotein
NCBI Official Symbol:
ORF1ab
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
Protein Accession #:
YP_009742609.1
Protein GI Number:
1826688921
Purification:
SARS-CoV-2 (COVID-19) NSP4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
Swissprot #:
P0DTC1
User NOte:
Optimal dilutions for each application to be determined by the researcher.