SARS-CoV-2 (COVID-19) NSP6 Antibody

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ProSci
Product Code: PSI-9177
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9177-0.02mg0.02mg£150.00
Quantity:
PSI-9177-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Virus
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
blue ice or RT
Storage:
SARS-CoV-2 (COVID-19) NSP6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

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<strong>Figure 1 ELISA Validation </strong><br> Antibodies: SARS-CoV-2 (COVID-19) NSP6 Antibody, 9177. A direct ELISA was performed using immunogen peptide (9177P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP6 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.
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<strong>Figure 1 ELISA Validation </strong><br> Antibodies: SARS-CoV-2 (COVID-19) NSP6 Antibody, 9177. A direct ELISA was performed using immunogen peptide (9177P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP6 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.

Documents

Further Information

Additional Names:
NSP6
Application Note:
Antibody validated: SARS-CoV-2 (COVID-19) NSP6 antibody can detect 2ng of free peptide at 1 μg/mL in ELISA.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP6 plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, it limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes (3,4).
Background References:
  • Gorbalenya. bioRxiv: 2020.
  • Hui et al. Int J Infect Dis. 2020;91:264-266.
  • Angelini et. al. DNA Cell Biol. 33:122-127 (2014)
  • Cottam et. al. Autophagy 2014; 10:1426-1441
Buffer:
SARS-CoV-2 (COVID-19) NSP6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV NSP6 protein: (94%)
Immunogen:
Anti-SARS-CoV-2 (COVID-19) NSP6 antibody (9177) was raised against a peptide corresponding to 18 amino acids in the center of SARS-CoV-2 (COVID-19) NSP6 protein.

The immunogen is located in between 230-280 amino acids of the SARS-CoV-2 (COVID-19) NSP6 protein.
NCBI Gene ID #:
43740578
NCBI Official Name:
ORF1a polyprotein;ORF1ab polyprotein
NCBI Official Symbol:
ORF1ab
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
Protein Accession #:
YP_009742613.1
Protein GI Number:
1826688923
Purification:
SARS-CoV-2 (COVID-19) NSP6 Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
Swissprot #:
P0DTC1
User NOte:
Optimal dilutions for each application to be determined by the researcher.