SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody
Code | Size | Price |
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PSI-9187-0.02mg | 0.02mg | £150.00 |
Quantity:
PSI-9187-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Virus
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
blue ice or RT
Storage:
SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Further Information
Additional Names:
Proofreading exoribonuclease, Guanine-N7 methyltransferase, Non-structural protein 14, NSP14.
Application Note:
Antibody validated: SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP14 (ExoN) is an enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity (3,4,5). It acts as a proofreading exoribonuclease for RNA replication, thereby lowering The sensitivity of the virus to RNA mutagens(6,7,8).
Background References:
- Gorbalenya. bioRxiv: 2020.
- Hui et al. Int J Infect Dis. 2020;91:264-266.
- Minskaia et. al. Proc. Natl. Acad. Sci. U.S.A 2006; 103:5108-5113
- Bouvet et. al. PLoS Pathog. 2010; 6:E1000863-E1000863
- Bouvet et. al. Proc. Natl. Acad. Sci. U.S.A. 2012;109:9372-9377
- Denison et. al. RNA Biol. 2011;8:270-279
- Smith et. al. PLoS Pathog. 2013;9:e1003565-e1003565
- Agostini et. al. MBio 2018; 9:0-0
Buffer:
SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV NSP14 (ExoN) protein: (79%).
Immunogen:
Anti-SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody (9187) was raised against a peptide corresponding to 14 amino acids near the center of SARS-CoV-2 (COVID-19) NSP14 (ExoN) protein.
The immunogen is located between 30-80 amino acids of SARS-CoV-2 (COVID-19) NSP14 (ExoN).
The immunogen is located between 30-80 amino acids of SARS-CoV-2 (COVID-19) NSP14 (ExoN).
NCBI Gene ID #:
43740578
NCBI Official Name:
ORF1a polyprotein;ORF1ab polyprotein
NCBI Official Symbol:
ORF1ab
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
Protein Accession #:
YP_009725309.1
Protein GI Number:
1802476817
Purification:
SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
Swissprot #:
P0DTC1
User NOte:
Optimal dilutions for each application to be determined by the researcher.