SARS-CoV-2 Spike NTD Antibody [2146] (HRP)

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ProSci
Product Code: PSI-10-057
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-10-057-0.05mg0.05mg£729.00
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Overview

Antibody Isotype: Human IgG1
Antibody Clonality: Recombinant Antibody
Antibody Clone: 2146
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Shipping:
blue ice or RT
Storage:
This horseradish peroxidase conjugated monoclonal antibody is stable when stored at 2-8˚ C. Do not freeze.

Images

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Coating: Purified Recombinant SARS-CoV-2 Spike RBD, concentration of 1 ul/well, 100 ul/well overnight 2-8˚C. Detection: Anti-SARS-CoV-2 NTD (Clone 2146) conjugated to HRP was serially diluted starting at 25 ng/ml down to 0.23 ng/ml, 100 ul/well for 1 hour at 37˚C. Substrate: TMB (Leinco T118), 100 ul/well for 15 min. at room temperature followed by 450 nm stop solution, 50ul/well.
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Coating: Purified Recombinant SARS-CoV-2 Spike RBD, concentration of 1 ul/well, 100 ul/well overnight 2-8˚C. Detection: Anti-SARS-CoV-2 NTD (Clone 2146) conjugated to HRP was serially diluted starting at 25 ng/ml down to 0.23 ng/ml, 100 ul/well for 1 hour at 37˚C. Substrate: TMB (Leinco T118), 100 ul/well for 15 min. at room temperature followed by 450 nm stop solution, 50ul/well.

Documents

Further Information

Additional Names:
SARS-CoV2 Spike NTD, COVID-19, 2019-nCoV, Severe acute respiratory syndrome coronavirus 2, SARS-CoV2
Application Note:
ELISA (Quality Tested by ProSci); IHC (Other applications as reported in literature)
Background References:
  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), is an enveloped, single-stranded, positive-sense RNA virus that belongs to the Coronaviridae family 1. The SARS-CoV-2 genome, which shares 79.6% identity with SARS-CoV, encodes four essential structural proteins: the spike (S), envelope (E), membrane (M), and nucleocapsid protein (N) 2. The S protein is a transmembrane, homotrimeric, class I fusion glycoprotein that mediates viral attachment, fusion, and entry into host cells 3. Each ~180 kDa monomer contains two functional subunits, S1 (~700 a.a) and S2 (~600 a.a), that mediate viral attachment and membrane fusion, respectively. S1 contains two major domains, the N-terminal (NTD) and C-terminal domains (CTD). In both SARS-CoV and SARS-CoV-2, the CTD contains the receptor-binding domain (RBD), which binds to the angiotensin-converting enzyme 2 (ACE2) receptor on host cells3-5. The NTD of SARS-CoV-2 does not bind to ACE26, and the function of NTD in SARS-CoV-2 infection is not well understood. In other CoVs, the NTD may promote attachment by binding to sugar moieties7 and might play a role in the conformational change of S2 required for membrane fusion8. While most neutralizing antibodies target the RBD domain and block receptor binding, potent neutralizing antibodies targeting NTD were isolated from convalescent COVID19 patients9, identifying the NTD as an attractive candidate for vaccines and therapeutics. In addition, the NTD is a promising antigen for diagnostic tests, as there is only 53.5% homology between the NTD of SARS-CoV-2 and SARS-CoV10.
  • Zhou, P., Yang, X., Wang, X. et al. Nature 579, 270?273. 2020.
  • Wu, F., Zhao, S., Yu, B. et al. Nature 579, 265?269. 2020.
  • Wrapp D, Wang N, Corbett KS, et al. bioRxiv. 2020.
  • Walls AC, Park YJ, Tortorici MA, et al. Cell. 181(2):281-292.e6. 2020.
  • Li W, Zhang C, Sui J, et al. EMBO J. 24(8):1634-1643. 2020.
  • Wang Q, Zhang Y, Wu L, et al. Cell. 181(4):894-904.e9. 2020.
  • Park, Y., Walls, A.C., Wang, Z. et al. Nat Struct Mol Biol 26, 1151?1157. 2019.
  • Zhou, H., Chen, Y., Zhang, S. et al. Nat Commun 10, 3068. 2019.
  • Chi X, Yan R, Zhang J, et al. Science. eabc6952. 2020.
Buffer:
This HRP-conjugated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 1% BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)
Concentration:
0.5 mg/ml
Conjugate:
HRP
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Sequenced from human survivors of COVID-19 (SARS-CoV-2). The spike NTD is expressed on the surface of SARS-CoV-2.
NCBI Official Name:
S
NCBI Official Symbol:
Optimal dilutions for each application to be determined by the researcher.
NCBI Organism:
surface glycoprotein
Physical State:
Liquid
Protein GI Number:
SARS-CoV-2
Research Area:
Infectious Disease,COVID-19
SPECIFICITY:
Anti-SARS-CoV-2 Spike NTD-HRP, clone 2146, specifically targets an epitope on the SARS-CoV-2 spike protein N-terminal domain. This antibody does not cross-react with the receptor-binding domain (RBD) of the spike protein or the NTD of SARS-CoV.
User NOte:
43740568

References

  1. Ou, X., Liu, Y., Lei, X. et al. Nat Commun 11, 1620. 2020.