SIGLEC15 Antibody

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ProSci
Product Code: PSI-9017
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9017-0.02mg0.02mg£150.00
Quantity:
PSI-9017-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
blue ice or RT
Storage:
SIGLEC15 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 Western Blot Validation in Human and Mouse Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Western Blot Validation in Human Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in Mouse Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 Western Blot Validation in Rat Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 1 Western Blot Validation in Human and Mouse Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Mouse Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Rat Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Documents

Further Information

Additional Names:
SIGLEC15 Antibody: CD33L3, HsT1361, SIGLEC-15, CD33L3, Sialic acid-binding Ig-like lectin 15, CD33 antigen-like 3, Siglec-15
Application Note:
WB: 2 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples. All other applications and species not yet tested.
Background:
SIGLEC15 Antibody: Siglecs are vertebrate cell-surface lectins that recognize sialylated glycans and are involved in many physiological processes, such as glycoprotein turnover, cellular trafficking, and pathogen recognition. Most Siglecs are expressed on cells of the immune system. SIGLEC15 is a type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain containing a lysine residue, and a short cytoplasmic tail. SIGLEC15 can interact with the activating adaptor molecules DAP12/10. Its activating signaling potential and unique preference for glycan recognition implies that SIGLEC15 may be involved in the immune surveillance of tumors and probably plays a conserved, regulatory role in the immune system of vertebrates.
Background References:
  • Crocker and Redelinghuys. Biochem. Soc. Trans. 2008; 36:1467-71.
  • Angata et al. Glycobiology 2007; 17:838-46.
  • Hiruma et al. Biochem. Biophys. Res. Commun. 2011; 409:424-9.
Buffer:
SIGLEC15 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti-SIGLEC15 antibody (9017) was raised against a peptide corresponding to 14 amino acids near the carboxy?terminus of human SIGLEC15.

The immunogen is located within amino acids 270-320 of SIGLEC15.
ISOFORMS:
Human SIGLEC15 has 2 isoforms, including isoform 1 (328aa, 36kD) and isoform 2 (174aa, 18kD). Mouse SIGLEC15 has one isoform (342aa, 37kD) and Rat SIGLEC15 also has one isoform (341aa, 37kD). 9017 can detect human and possibly mouse and rat isoforms.
NCBI Gene ID #:
284266
NCBI Official Name:
sialic acid binding Ig-like lectin 15
NCBI Official Symbol:
SIGLEC15
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 36kD

Observed: 34-36kD
Protein Accession #:
NP_998767
Protein GI Number:
47106069
Purification:
SIGLEC15 Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
Swissprot #:
Q6ZMC9
User NOte:
Optimal dilutions for each application to be determined by the researcher.