SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody
| Code | Size | Price |
|---|
| PSI-9151-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-9151-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Virus
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
blue ice or RT
Storage:
SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody can be stored at 4 ˚ C for three months and -20 ˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
3C-like proteinase, 3CLp, nsp5, Non-structure protein 5, Main protease, Mpro
Application Note:
WB: 0.5 μg/mL
Antibody validated: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA.
Antibody validated: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. 3C- like proteinase (NSP5) cleaves the C-terminus of replicase polyprotein at 11 sites (3,4,5). It recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. It also able to bind an ADP-ribose-1''-phosphate (ADRP) (5).
Background References:
- Gorbalenya. bioRxiv: 2020.
- Hui et al. Int J Infect Dis. 2020;91:264-266.
- Zhang et al. Science. 2020; 0:0-0(2020)
- Jin et al. Nature, 0:0-0(2020)
- Dai et al. Science 0:0-0(2020)
Buffer:
SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV 3CL-PRO (NSP5) protein: (87%)
Immunogen:
Anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody (9151) was raised against a peptide corresponding to 15 amino acids in the center of SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) protein.
The immunogen is located within 80-130 amino acids of the SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) protein.
The immunogen is located within 80-130 amino acids of the SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) protein.
NCBI Gene ID #:
43740578
NCBI Official Name:
ORF1a polyprotein;ORF1ab polyprotein
NCBI Official Symbol:
ORF1ab
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
Protein Accession #:
YP_009742612.1
Protein GI Number:
1826688922
Purification:
SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
Swissprot #:
P0DTC1
User NOte:
Optimal dilutions for each application to be determined by the researcher.