SARS-CoV-2 (COVID-19) ORF9b Antibody

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ProSci
Product Code: PSI-9191
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9191-0.02mg0.02mg£150.00
Quantity:
PSI-9191-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
blue ice or RT
Storage:
SARS-CoV-2 (COVID-19) ORF9b antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

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<strong>Figure 1 ELISA Validation </strong><br> Antibodies: SARS-CoV-2 (COVID-19) ORF9b Antibody, 9191. A sandwich ELISA was performed using immunogen peptide (9191P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF9b antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.
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<strong>Figure 1 ELISA Validation </strong><br> Antibodies: SARS-CoV-2 (COVID-19) ORF9b Antibody, 9191. A sandwich ELISA was performed using immunogen peptide (9191P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF9b antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.

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Further Information

Additional Names:
Accessory protein 9b, ORF-9b, Protein 9b
Application Note:
WB: 2 μg/mL

Antibody validated: SARS-CoV-2 (COVID-19) ORF9b antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. ORF9b plays a role in the inhibition of host innate immune response by targeting the mitochondrial-associated adapter MAVS. Mechanistically, it usurps the E3 ligase ITCH to trigger the degradation of MAVS, TRAF3, and TRAF6. In addition, it causes mitochondrial elongation by triggering ubiquitination and proteasomal degradation of dynamin-like protein 1/DNM1L(3).
Background References:
  • Gorbalenya. bioRxiv: 2020.
  • Hui et al. Int J Infect Dis. 2020;91:264-266.
  • Shi. et al. J. Immunol 2014; 193:3090-3089
Buffer:
SARS-CoV-2 (COVID-19) ORF9b Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV ORF9b proteins: (50%)
Immunogen:
Anti-SARS-CoV-2 (COVID-19) ORF9b antibody (9191) was raised against a peptide corresponding to 14 amino acids near the amino terminus of SARS-CoV-2 (COVID-19) ORF9b protein.

The immunogen is located in the first 50 amino acids of the SARS-CoV-2 (COVID-19) ORF9b protein.
NCBI Official Symbol:
ORF9b
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
Protein Accession #:
P0DTD2.1
Protein GI Number:
1835921959
Purification:
SARS-CoV-2 (COVID-19) ORF9b Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
User NOte:
Optimal dilutions for each application to be determined by the researcher.