SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody

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ProSci
Product Code: PSI-9183
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9183-0.02mg0.02mg£150.00
Quantity:
PSI-9183-0.1mg0.1mg£449.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
blue ice or RT
Storage:
SARS-CoV-2 (COVID-19) NSP13 (Helicase) antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP13 in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP13 antibody (9183, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP13 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
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<strong>Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) NSP13 (Helicase) Protein </strong><br> Antibodies: SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody, 9183. A direct ELISA was performed using SARS-CoV-2 NSP13 recombinant protein (10-427) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP13 (Helicase) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.
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<strong>Figure 3 ELISA Validation </strong><br> Antibodies: SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody, 9183. A direct ELISA was performed using immunogen peptide (9183P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP13 (Helicase) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL
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<strong>Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP13 in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP13 antibody (9183, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP13 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
<strong>Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) NSP13 (Helicase) Protein </strong><br> Antibodies: SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody, 9183. A direct ELISA was performed using SARS-CoV-2 NSP13 recombinant protein (10-427) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP13 (Helicase) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.
<strong>Figure 3 ELISA Validation </strong><br> Antibodies: SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody, 9183. A direct ELISA was performed using immunogen peptide (9183P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP13 (Helicase) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL

Documents

Further Information

Additional Names:
Helicase, Non-structural protein 13, NSP13
Application Note:
IHC: 0.5 μg/mL.

Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) NSP13 antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 NSP13 recombinant protein by ELISA. All other applications and species not yet tested
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP13 is a multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of Helicase is dependent on magnesium(3,4).
Background References:
  • Gorbalenya. bioRxiv: 2020.
  • Hui et al. Int J Infect Dis. 2020;91:264-266.
  • Adedeji et. al. PLoS ONE 2012; 7:E36521-E36521
Buffer:
SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV NSP13 (Helicase) protein: (80%)
Immunogen:
Anti-SARS-CoV-2 (COVID-19) NSP13 (Helicase)antibody (9183) was raised against a peptide corresponding to 14 amino acids near the center of the SARS-CoV-2 (COVID-19) NSP13 (Helicase) protein.

The immunogen is located between 440-490 amino acids of SARS-CoV-2 (COVID-19) NSP13 (Helicase).
NCBI Gene ID #:
43740578
NCBI Official Name:
ORF1a polyprotein;ORF1ab polyprotein
NCBI Official Symbol:
ORF1ab
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
Protein Accession #:
YP_009725308.1
Protein GI Number:
1802476816
Purification:
SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
Swissprot #:
P0DTC1
User NOte:
Optimal dilutions for each application to be determined by the researcher.