SARS-CoV-2 (COVID-19) NSP8 Antibody

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ProSci
Product Code: PSI-9159
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9159-0.02mg0.02mg£150.00
Quantity:
PSI-9159-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
blue ice or RT
Storage:
SARS-CoV-2 (COVID-19) NSP8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP8 in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP8 antibody (9159, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP8 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
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<strong>Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) NSP8 Protein </strong><br> Antibodies: SARS-CoV-2 (COVID-19) NSP8 Antibody, 9159. A direct ELISA was performed using SARS-CoV-2 NSP8 recombinant protein (10-415) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP8 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL
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<strong>Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP8 in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP8 antibody (9159, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP8 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
<strong>Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) NSP8 Protein </strong><br> Antibodies: SARS-CoV-2 (COVID-19) NSP8 Antibody, 9159. A direct ELISA was performed using SARS-CoV-2 NSP8 recombinant protein (10-415) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP8 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL

Documents

Further Information

Additional Names:
NSP8
Application Note:
IHC: 0.5 μg/ml.

Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) NSP8 antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 NSP8 recombinant protein by ELISA. All other applications and species not yet tested.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP8 plays a role in viral RNA synthesis (3,4,5). Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, it may synthesize substantially longer products than oligonucleotide primers (6).
Background References:
  • Gorbalenya. bioRxiv: 2020.
  • Hui et al. Int J Infect Dis. 2020;91:264-266.
  • Gao et. al Science 2020;0:0-0
  • Yin et. al Science 2020;0:0-0
Buffer:
SARS-CoV-2 (COVID-19) NSP8 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV NSP8 protein: (100%)
Immunogen:
Anti-SARS-CoV-2 (COVID-19) NSP8 antibody (9159) was raised against a peptide corresponding to 14 amino acids near the center of SARS-CoV-2 (COVID-19) NSP8 protein.

The immunogen is located within 60-110 amino acids of SARS-CoV-2 (COVID-19) NSP8.
NCBI Gene ID #:
43740578
NCBI Official Name:
ORF1a polyprotein;ORF1ab polyprotein
NCBI Official Symbol:
ORF1ab
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
Protein Accession #:
YP_009742615.1
Protein GI Number:
1826688925
Purification:
SARS-CoV-2 (COVID-19) NSP8 Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
Swissprot #:
P0DTC1
User NOte:
Optimal dilutions for each application to be determined by the researcher.