SARS-CoV-2 (COVID-19) NSP9 Antibody

Product Code: PSI-9163

Supplier: ProSci

Code	Size	Price

PSI-9163-0.02mg

0.02mg

£150.00

Quantity:

PSI-9163-0.1mg

0.1mg

£449.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species: Virus

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Western Blot (WB)

Shipping:

blue ice or RT

Storage:

SARS-CoV-2 (COVID-19) NSP9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) NSP9 Protein </strong><br>
Antibodies: SARS-CoV-2 (COVID-19) NSP9 Antibody, 9163. A direct ELISA was performed using SARS-CoV-2 NSP9 recombinant protein (10-417) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP9 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 3000 ng/mL

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<strong>Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) NSP9 Protein</strong><br>
Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP9 recombinant protein (10-417).
Antibodies: SARS-CoV-2 (COVID-19) NSP9, 9163, 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Lane 1: 0.5 μg/mL and
Lane 2: 1 μg/mL

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Documents

Further Information

Additional Names:

NSP9

Application Note:

WB: 0.5 μg/mL

Antibody validated: SARS-CoV-2 (COVID-19) NSP9 antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA.

Background:

Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP9 may participate in viral replication by acting as a ssRNA-binding protein (3).

Background References:

Gorbalenya. bioRxiv: 2020.
Hui et al. Int J Infect Dis. 2020;91:264-266.
Miknis et. al. J.Virol. 2009;83:3007-3018

Buffer:

SARS-CoV-2 (COVID-19) NSP9 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Predicted reactivity based on immunogen sequence: SARS-CoV NSP9 protein: (100%)

Immunogen:

Anti-SARS-CoV-2 (COVID-19) NSP9 antibody (9163) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of SARS-CoV-2 (COVID-19) NSP9 protein.

The immunogen is located within the last 50 amino acids of the SARS-CoV-2 (COVID-19) NSP9 protein

NCBI Gene ID #:

43740578

NCBI Official Name:

ORF1a polyprotein;ORF1ab polyprotein

NCBI Official Symbol:

ORF1ab

NCBI Organism:

Severe acute respiratory syndrome coronavirus 2

Physical State:

Liquid

Protein Accession #:

YP_009742616.1

Protein GI Number:

1826688926

Purification:

SARS-CoV-2 (COVID-19) NSP9 Antibody is affinity chromatography purified via peptide column.

Research Area:

Infectious Disease,COVID-19

Swissprot #:

P0DTC1

User NOte:

Optimal dilutions for each application to be determined by the researcher.