SARS-CoV-2 (COVID-19) Nucleoprotein Antibody [85E9]

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ProSci
Product Code: PSI-11-021
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-11-021-0.1mg0.1mg£657.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG1k
Antibody Clonality: Monoclonal
Antibody Clone: [85E9]
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
SARS-CoV-2 (COVID-19) Nucleoprotein Antibody [85E9] can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) and SARS-CoV NP at 1 ug/mL. Purified rabbit monoclonal antibody 85E9 was serially diluted 1:2 starting at 1 ug/mL. Rabbit monoclonal antibody 85E9 shows very strong and specific binding to both COVID-19 NP antigen and recognizes SARS-CoV NP antigen.
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Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) and SARS-CoV NP at 1 ug/mL. Purified rabbit monoclonal antibody 85E9 was serially diluted 1:2 starting at 1 ug/mL. Rabbit monoclonal antibody 85E9 shows very strong and specific binding to both COVID-19 NP antigen and recognizes SARS-CoV NP antigen.

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Further Information

Additional Names:
SARS-CoV-2 (COVID-19) Nucleoprotein Antibody [85E9]: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Application Note:
ELISA
Background:
We have leveraged our next-generation rabbit mAb discovery platform to develop recombinant rabbit monoclonal antibodies with extremely high sensitivity and specificity to SARS-CoV-2. These antibodies, which include clones 75G5a, 84C4a, 84D7, 85C1, 85C10, 85B4, and 85E9 can be paired in sandwich detection assay and used to detect nucleoprotein (NP) antigen from SARS-CoV-2. Antibodies 75G5a, 84C4a, 84D7, 85C10, and 85B4 do not cross-react to the highly related SARS-CoV virus or to any other coronaviruses tested. Sandwich ELISA detection using TMB/acid developer reliably detects NP antigen in the pg/mL range, and sensitivity is expected to be even higher when using more sensitive developer strategies. This panel of antibodies can be used as raw materials for diagnostic kits and can be applied to lateral flow systems for diagnostic detection of COVID-19.
Buffer:
1X PBS, 0.02% NaN3
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Highly-specific to SARS-CoV-2 nucleoprotein; does not cross-react with nucleoprotein from SARS-CoV or other coronaviruses
Immunogen:
SARS-CoV-2 nucleoprotein (NP) specific peptide
NCBI Gene ID #:
43740575
NCBI Official Name:
Nucleocapsid Phosphoprotein
NCBI Official Symbol:
N
NCBI Organism:
SARS-CoV-2
Physical State:
Liquid
Protein Accession #:
QHD43423
Protein GI Number:
1820552696
Purification:
SARS-CoV-2 (COVID-19) Nucleoprotein Antibody [85E9] is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
User NOte:
Optimal dilutions for each application to be determined by the researcher.