SARS-CoV-2 (COVID-19) Membrane Antibody

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ProSci
Product Code: PSI-9157
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9157-0.02mg0.02mg£150.00
Quantity:
PSI-9157-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
blue ice or RT
Storage:
SARS-CoV-2 (COVID-19) Membrane antibody can be stored at 4 ˚ C for three months and -20 ˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Membrane in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Membrane antibody (9157, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Membrane protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
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<strong>Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) Membrane Protein </strong><br> Antibodies: SARS-CoV-2 (COVID-19) Membrane Antibody, 9157. A direct ELISA was performed using SARS-CoV-2 membrane recombinant protein (10-429) as coating antigen and the anti-SARS-CoV-2 (COVID-19) Membrane antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/ml
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<strong>Figure 3 Western Blot Validation with SARS-CoV-2 (COVID-19) Membrane Protein</strong><br> Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) membrane protein (10-429). Antibodies: SARS-CoV-2 (COVID-19) membrane, 9157, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 μg/mL and Lane 2: 0.5 μg/mL
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<strong>Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Membrane in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Membrane antibody (9157, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Membrane protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
<strong>Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) Membrane Protein </strong><br> Antibodies: SARS-CoV-2 (COVID-19) Membrane Antibody, 9157. A direct ELISA was performed using SARS-CoV-2 membrane recombinant protein (10-429) as coating antigen and the anti-SARS-CoV-2 (COVID-19) Membrane antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/ml
<strong>Figure 3 Western Blot Validation with SARS-CoV-2 (COVID-19) Membrane Protein</strong><br> Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) membrane protein (10-429). Antibodies: SARS-CoV-2 (COVID-19) membrane, 9157, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 μg/mL and Lane 2: 0.5 μg/mL

Documents

Further Information

Additional Names:
SARS-CoV-2/SARS-CoV Matrix Antibody: SARS-CoV-2 Matrix protein, SARS Matrix protein, Membrane protein, E1 glycoprotein, M protein
Application Note:
WB: 0.25 μg/mL; IHC: 0.5 μg/mL

Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) Membrane antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 Membrane recombinant protein by ELISA and WB. All other applications and species not yet tested.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019?20 coronavirus outbreak (1). SARS-CoV-2 is the seventh member of the enveloped, positive-stranded RNA viruses that are able to infect humans. The SARS-CoV-2 genome, like other coronaviruses, encodes for multiple structural and nonstructural proteins. The structural proteins include spike protein (S), envelope protein (E), membrane glycoprotein (M), nucleocapsid phosphoprotein (N), and the nonstructural proteins include open reading frame 1ab (ORF1ab), ORF3a, ORF6, ORF7a, ORF8, and ORF10 (2).The membrane (M) protein or matrix protein is the most abundant structural protein and defines the shape of the viral envelope (3). It is an integral membrane protein involved in the budding of the viral particles and interacts with S (Spike) protein. It involves in organization of the nucleoprotein inside, which includes many copies of the N (nucleocapsid) protein bound to the genomic RNA. The M protein holds dominant cellular immunogenicity and has been determined as a protective antigen in humoral responses, which suggests it would serve as a potential target in vaccine design (4).
Background References:
  • Zhu et al. N Engl J Med. 2020 Feb 20;382(8):727-733.
  • Kiyotani et al. J Hum Genet. 2020 Jul;65(7):569-575
  • Neuman et al. J Struct Biol. 2011;174(1):11?22.
  • Liu et al. J Infect Dis. 2010;202(8):1171-80.
Buffer:
SARS-CoV-2 (COVID-19) Membrane Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV Matrix protein: (100%)
Immunogen:
Anti-SARS-CoV-2 (COVID-19) Membrane antibody (9157) was raised against a peptide corresponding to 13 amino acids near the center of SARS-CoV-2 (COVID-19) Membrane protein.

The immunogen is located between 130-180 amino acids of the SARS-CoV-2 (COVID-19) Membrane protein.
NCBI Gene ID #:
43740571
NCBI Official Name:
Membrane protein
NCBI Official Symbol:
M
Physical State:
Liquid
Protein Accession #:
QJW69073
Protein GI Number:
1800455127
Purification:
SARS-CoV-2 (COVID-19) Membrane Antibody is affinity chromatography
Research Area:
Infectious Disease,COVID-19
User NOte:
Optimal dilutions for each application to be determined by the researcher.