IL-33 Antibody [IL33305B]
| Code | Size | Price |
|---|
| PSI-36-355-50ug | 50ug | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a?
Antibody Clonality: Monoclonal
Antibody Clone: IL33305B
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Functional Study
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
blue ice or RT
Storage:
Stable for at least 1 year after receipt when stored at -20˚C.
Documents
Further Information
Additional Names:
Interleukin-33; IL-1F11; NF-HEV
Application Note:
ELISA: (direct or indirect: 1:2'000-1:5'000), Functional Application (Blocking), Immunohistochemistry (paraffin sections (1:50-1:200)), Immunoprecipitation Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
Background:
Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38(MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.
Buffer:
Liquid. 0.2?m-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human IL-33.
Physical State:
Liquid
Purification:
Protein G-affinity purified.
Research Area:
Chemokines & Cytokines
SPECIFICITY:
Recognizes human IL-33. Does not cross-react with mouse IL-33.
User NOte:
Optimal dilutions for each application to be determined by the researcher.