SARS-CoV-2 Nucleocapsid Antibody

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ProSci
Product Code: PSI-10-065
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-10-065-0.1mg0.1mg£686.00
Quantity:
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Overview

Antibody Isotype: Mouse IgG2a
Antibody Clonality: Recombinant Antibody
Antibody Clone: 1C7C7
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
blue ice or RT
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8˚ C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80˚ C. Avoid Repeated Freeze Thaw Cycles.

Images

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Vero E6 infectedcwith SARS-CoV-2, Wa-1 @ MOI = 0.1 for 48 hours. Cells were fixed with 4 % formaldehyde (4hr), perm 0.1% Triton X (15min), blocked with 4 % BSA, 1:1000 diluted with clone 1C7C7 (Anti-SARS-CoV-2 Nucleocaspid) O/N tehn 1:1000 anti-mouse Dylight 488 (1 hour), Image Courtesy of Dr. Ana Fernandex - Sesma Laboratory, ISMMS.
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Lysates were derived from A549 cells (Transduced with ACE2 by adenoviral vector), mock vs. SARS-CoV-2-infected MO12, 24 hours, with 60ug of lysates loaded/lane, Image Courtesy of Jeff Johnson Laboratory, ISMMS.
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Exp1293 cells were transfected with expression plasmid coding for SARs2 NP and incubated for 48 hours. Cells were permeabilized, incubated with dilutions of supermatant collected from cells transfected with DNA plasmids coding for the heavy and light chains of 1C7 and binding identified by the addition of goat anti-mouse IgG FITC.
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Vero E6 infectedcwith SARS-CoV-2, Wa-1 @ MOI = 0.1 for 48 hours. Cells were fixed with 4 % formaldehyde (4hr), perm 0.1% Triton X (15min), blocked with 4 % BSA, 1:1000 diluted with clone 1C7C7 (Anti-SARS-CoV-2 Nucleocaspid) O/N tehn 1:1000 anti-mouse Dylight 488 (1 hour), Image Courtesy of Dr. Ana Fernandex - Sesma Laboratory, ISMMS.
Lysates were derived from A549 cells (Transduced with ACE2 by adenoviral vector), mock vs. SARS-CoV-2-infected MO12, 24 hours, with 60ug of lysates loaded/lane, Image Courtesy of Jeff Johnson Laboratory, ISMMS.
Exp1293 cells were transfected with expression plasmid coding for SARs2 NP and incubated for 48 hours. Cells were permeabilized, incubated with dilutions of supermatant collected from cells transfected with DNA plasmids coding for the heavy and light chains of 1C7 and binding identified by the addition of goat anti-mouse IgG FITC.

Documents

Further Information

Additional Names:
SARS-CoV-2 Nucleocapsid, SARS-CoV-2 Nucleoprotein, Protein N, SARS-CoV N Protein
Application Note:
ELISA, IHC, WB (Quality Tested by ProSci); FC (Other applications as reported in literature)
Background References:
  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), is an enveloped, single-stranded, positive-sense RNA virus that belongs to the Coronaviridae family 1. The SARS-CoV-2 genome, which shares 79.6% identity with SARS-CoV, encodes four essential structural proteins: the spike (S), envelope (E), membrane (M), and nucleocapsid protein (N) 2. The N protein is 46 kDa and consists of two highly conserved structural domains, the N-terminal domain (NTD) and C-terminal domain (CTD), connected by a linker region. The NTD and CTD are involved in RNA binding and self-oligomerization, respectively 3, 4. The primary function of the N protein is to bind to and package the viral RNA genome into a helical ribonucleoprotein complex 5. The N protein is also involved in other critical steps of the viral life cycle, including transcription, replication, and modulating infected cell signaling pathways 6, 7. The N protein is abundantly expressed during infection and is highly conserved, sharing 90% amino acid homology with the SARS-CoV N protein 8. It is also immunogenic, and antibodies 8,9 and memory T cells 10, 11 targeting the N protein are present in the sera of convalescent COVID-19 patients, identifying the N protein as a suitable candidate for vaccine development and diagnostic assays. Diagnostic assays based on the N protein effectively detect antibodies in the sera of patients infected with SARS-CoV-2 12. The N protein also contributes to immune evasion by antagonizing antiviral RNAi 13, suggesting its potential value as a targeted therapeutic.
  • Zhou, P., Yang, X., Wang, X. et al. Nature 579, 270?273. 2020.
  • Wu, F., Zhao, S., Yu, B. et al. Nature 579, 265?269. 2020.
  • Kang S, Yang M, Hong Z, et al. Acta Pharm Sin B. 10.1016/j.apsb.2020.04.009. 2020.
  • Chang CK, Sue SC, Yu TH, et al. J Biomed Sci. 13(1):59-72. 2006.
  • Hsieh PK, Chang SC, Huang CC, et al. J Virol. 79(22):13848-13855. 2005.
  • Surjit M, Lal SK. Infect Genet Evol. 8(4):397-405. 2008.
  • Hurst KR, Ye R, Goebel SJ, Jayaraman P, Masters PS. J Virol. 84(19):10276-10288. 2010.
  • Guo L., Ren L., Yang S., et al. Clinical Infectious Diseases: an Official Publication of the Infectious Diseases Society of America. 2020.
  • To K.K., Tsang O.T., Leung W.S., et al. Lancet Infect. Dis. 2020.
Buffer:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added.
Concentration:
>1.0 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant nucleoprotein of SARS-CoV (severe acute respiratory syndrome).The nucleocapsid protein is expressed in the internal nucleocapsid of SARS-CoV-2.
NCBI Official Name:
N
NCBI Official Symbol:
Optimal dilutions for each application to be determined by the researcher.
NCBI Organism:
nucleocapsid phosphoprotein
Physical State:
Liquid
Protein GI Number:
SARS-CoV-2
Purification:
>95% by SDS Page
Research Area:
Infectious Disease,COVID-19
SPECIFICITY:
Anti-SARS-CoV-2 Nucleocapsid, clone 1C7C7, specifically targets an epitope on the SARS-CoV-2 and SARS-CoV nucleocapsid protein. This antibody does not cross-react with the nucleocapsid of other common human coronaviruses, OC43 and 229E
User NOte:
43740575

References

  1. Ni L, Ye F, Cheng ML, et al. Immunity. 52(6):971-977.e3. 2020.