SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [NP642]

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ProSci
Product Code: PSI-10-352
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-10-352-0.1mg0.1mg£638.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG, kappa
Antibody Clonality: Monoclonal
Antibody Clone: NP642
Regulatory Status: RUO
Target Species: Virus
Shipping:
blue ice or RT
Storage:
SARS-CoV-2 (COVID-19) Nucleocapsid antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Nucleocapsid antibody (10-352, 1 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
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<strong>Figure 3 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies</strong><br> Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, 10-352 and 35-720. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (10-352, 5ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (10-306), and the anti-SARS-CoV-2 Nucleocapsid antibody (35-720, 1ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 2.36 ng
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<strong>Figure 4 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies</strong><br> Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, 10-352 and 35-722. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (10-352, 5ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (10-306), and the anti-SARS-CoV-2 Nucleocapsid antibody (35-722, 1ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 2.7 ng
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<strong>Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Nucleocapsid antibody (10-352, 1 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
<strong>Figure 3 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies</strong><br> Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, 10-352 and 35-720. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (10-352, 5ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (10-306), and the anti-SARS-CoV-2 Nucleocapsid antibody (35-720, 1ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 2.36 ng
<strong>Figure 4 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies</strong><br> Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, 10-352 and 35-722. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (10-352, 5ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (10-306), and the anti-SARS-CoV-2 Nucleocapsid antibody (35-722, 1ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 2.7 ng

Documents

Further Information

Additional Names:
SARS-CoV-2 (COVID-19) Nucleocapsid antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Nucleocapsid protein, N protein, Nucleocapsid Phosphoprotein
Buffer:
PBS pH7.2
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant SARS-CoV-2 nucleocapsid protein (full-length).
NCBI Gene ID #:
43740575
NCBI Official Name:
Nucleocapsid Phosphoprotein
NCBI Official Symbol:
N
NCBI Organism:
SARS-CoV-2
Physical State:
Liquid
Protein Accession #:
QHD43423
Protein GI Number:
1820552696
Purification:
The antibody was expressed in HEK293F cells, and subsequently obtained from cell supernatant by protein A affinity chromatography.
Research Area:
Infectious Disease,COVID-19
User NOte:
Optimal dilutions for each application to be determined by the researcher.