SARS-CoV-2 (COVID-19) Spike S1 Antibody [SP185]

Product Code: PSI-10-351

Supplier: ProSci

Code	Size	Price

PSI-10-351-0.1mg

0.1mg

£638.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG, kappa

Antibody Clonality: Monoclonal

Antibody Clone: SP185

Regulatory Status: RUO

Target Species: Virus

Shipping:

blue ice or RT

Storage:

SARS-CoV-2 (COVID-19) Spike S1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong> Figure 1 Detection of SARS-CoV-2 Variant Proteins with Spike S1 Antibodies by Direct ELISA</strong>
Coating Antigen: SARS-CoV-2 full length spike proteins, including WT, UK variant (B.1.1.7), SA variant (B.1.135) and Brazil (P.1). Dilution: 1-3000 ng/mL. Incubate at 4 ˚ C overnight.
Detection Antibodies: SARS-CoV-2 Spike S1 Antibody, 10-351, 0.1 μg/mL, incubate at RT for 1 hr.
Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.
<strong>Spike S1 antibody (10-351) cannot detect SA variant (B.1.135) and Brazil (P.1), both of which carry more mutations in RBD as compared to UK variant (B.1.1.7). </strong>

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<strong>Figure 3 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies</strong><br>
Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, 10-351 and 10-706. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (10-351, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (97-087), and the anti-SARS-CoV-2 Spike S1 antibody (10-706, 1ug/ml) as the detection antibody. Secondary: Mouse anti-human IgG HRP conjugate (PM6727) at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.58 ng

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<strong>Figure 4 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies</strong><br>
Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, 10-351 and 9083-biotin. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (10-351, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (97-087), and the anti-SARS-CoV-2 Spike S1 antibody (9083-biotin, 1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.96 ng

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<strong>Figure 5 Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies</strong><br>
Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, 10-351 and 9091-biotin. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (10-351, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (97-087), and the anti-SARS-CoV-2 Spike S1 antibody (9091-biotin, 1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 3.14 ng

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Documents

Further Information

Additional Names:

SARS-CoV-2 (COVID-19) Spike S1 Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein

Buffer:

PBS pH7.2

Concentration:

batch dependent

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

Recombinant SARS-CoV-2 spike protein, S1 subunit.

NCBI Gene ID #:

43740568

NCBI Official Name:

surface glycoprotein

NCBI Official Symbol:

NCBI Organism:

SARS-CoV-2

Physical State:

Liquid

Protein Accession #:

QHD43416

Protein GI Number:

1791269090

Purification:

The antibody was expressed in HEK293F cells, and subsequently obtained from cell supernatant by protein A affinity chromatography.

Research Area:

Infectious Disease,COVID-19

User NOte:

Optimal dilutions for each application to be determined by the researcher.