DYKDDDDK tag monoclonal antibody, clone 29E4.G7 (HRP)
| Code | Size | Price |
|---|
| MAB23148-100ug | 100 ug | £597.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a, kappa
Antibody Clone: 29E4.G7
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store vial at 4°C prior to restoration.
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Documents
Further Information
Conjugate Tag:
HRP
Form:
Lyophilized
Immunogen:
A synthetic peptide (conjugated with KLH) corresponding to FLAG epitope tag.
Immunogen Sequence Protein Sequence:
DYKDDDDK
Preparation Method:
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Product Description:
Mouse monoclonal antibody raised against synthetic peptides of FLAG epitope tag. The antibody is conjugated with Horseradish Peroxidase (HRP).
Recommend Dilutions:
ELISA (1:10000-1:50000)
Immunohistochemistry (1:500-1:2500)
Western Blot (1:10000)
The optimal working dilution should be determined by the end user.
Immunohistochemistry (1:500-1:2500)
Western Blot (1:10000)
The optimal working dilution should be determined by the end user.
Specificity:
Carboxy and amino terminal linked FLAG tagged recombinant proteins. This antibody is directed against the FLAG epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the FLAG epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Storage Buffer:
Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) gentamicin sulfate). Do NOT add sodium azide!