DYKDDDDK tag polyclonal antibody (Biotin)
| Code | Size | Price |
|---|
| PAB31809-100ug | 100 ug | £709.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store vial at 4°C prior to restoration.
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Documents
Further Information
Conjugate Tag:
Biotin
Form:
Lyophilized
Immunogen:
A synthetic peptide (conjugated with KLH) corresponding to DYKDDDDK tag.
Immunogen Sequence Protein Sequence:
DYKDDDDK
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Preparation Method:
This antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the carboxy terminal end to facilitate coupling. This antibody reacts with FLAG conjugated proteins.
Product Description:
Rabbit polyclonal antibody raised against synthetic peptides of FLAG epitope tag. The antibody is conjugated with Biotin.
Recommend Dilutions:
ELISA (1:10000-1:50000)
Immunohistochemistry (1:1000-1:5000)
Western Blot (1:2000-1:10000)
The optimal working dilution should be determined by the end user.
Immunohistochemistry (1:1000-1:5000)
Western Blot (1:2000-1:10000)
The optimal working dilution should be determined by the end user.
Specificity:
Carboxy and amino terminal linked FLAG tagged recombinant proteins. This antibody can be used to identify fusion proteins containing the FLAG epitope. The antibody recognizes the epitope tag fused to the carboxy or amino- terminus of targeted proteins. This affinity purified antibody is directed against the FLAG motif and is useful in determining its presence in various assays. This polyclonal antibody detects over-expressed proteins containing the FLAG epitope tag. To date this antibody has reacted with all amino-terminal FLAG tagged proteins so far tested. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins.
Storage Buffer:
Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) sodium azide).