MYC tag polyclonal antibody (HRP)
| Code | Size | Price |
|---|
| PAB31805-100ug | 100 ug | £709.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store vial at 4°C prior to restoration.
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Documents
Further Information
Conjugate Tag:
HRP
Form:
Lyophilized
Immunogen:
A epitope tag peptide (conjugated with KLH) corresponding to amino acids 410-419 of human c-MYC.
Immunogen Sequence Protein Sequence:
EQKLISEEDL
Preparation Method:
This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
Product Description:
Rabbit polyclonal antibody raised against human MYC epitope tag peptide. The antibody is conjugated with Horseradish peroxidase (HRP).
Recommend Dilutions:
ELISA (1:10000-1:50000)
Immunohistochemistry (1:500-1:2000)
Western Blot (1:2000-1:5000)
The optimal working dilution should be determined by the end user.
Immunohistochemistry (1:500-1:2000)
Western Blot (1:2000-1:5000)
The optimal working dilution should be determined by the end user.
Specificity:
This affinity purified antibody is directed against human c-myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-rabbit serum.
Storage Buffer:
Lyophilized from 20 mM potassium phosphate, 150 mM NaCl, pH 7.2 (10 mg/mL BSA, 0.01% (w/v) Gentamicin Sulfate). Do NOT add sodium azide!