Goat Anti-Mouse IgG F(c) secondary antibody (HRP)
| Code | Size | Price |
|---|
| PAB31797-20mg | 20 mg | £597.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store vial at 4°C prior to restoration.
After reconstitution with 2.0 mL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
After reconstitution with 2.0 mL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Documents
Further Information
Antigen Target Species:
Mouse
Conjugate Tag:
HRP
Form:
Lyophilized
Immunogen:
Mouse IgG F(c) fragment.
Product Description:
Goat polyclonal antibody raised against mouse IgG F(c) fragment. This secondary antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. The antibody is conjugated with Horseradish Peroxidase (HRP).
Recommend Dilutions:
ELISA (1:10000-1:50000)
Immunohistochemistry (1:500-1:2500)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.
Immunohistochemistry (1:500-1:2500)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.
Specificity:
IgG F(c). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-goat serum, mouse IgG, mouse IgG F(c) and mouse serum. No reaction was observed against mouse IgG F(ab).
Storage Buffer:
Lyophilized from 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) gentamicin sulfate). Do NOT add sodium azide!
References
- https://www.ncbi.nlm.nih.gov/pubmed/34584879