anti-Crf2 (Aspergillus fumigatus) mAb (rec.) (MS112-IIA1)

Chitin Ring Formation 2; Crh-like Protein 2

1 mg/ml

32160000

liquid

Liquid. In PBS.

After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Please handle under sterile conditions to avoid contamination.

Recombinant Crf2 (Aspergillus fumigatus) containing a C-terminal His-tag.

Recombinant Antibody. Recognizes Crf2 from Aspergillus fumigatus. Possible cross-reactivity with Crf1, Crf2 and Asp f9 [Reference 2]. Applications: ELISA, FUNC, IHC, WB, IF. Clone: MS112-IIA1. Isotype: Human IgG1. Formulation: Liquid. In PBS. Aspergillus fumigatus is a common airborne fungal pathogen for humans. It frequently causes an invasive aspergillosis (IA) in immunocompromised patients with poor prognosis. Potent antifungal drugs are very expensive and cause serious adverse effects. Their correct application requires an early and specific diagnosis of IA. Encoded by CRF1 gene, the Crf protein family contains three protein variants: Crf2 protein, amino acids (333 aa) long, Asp f9 protein (292 aa), first identified as an allergen using serum from Aspergillus-allergic patients and Crf1 (395 aa, also named Crh5). The glycosyol hydrolase/glycosidase Crf2 catalyzes the hydrolysis of glycosidic bonds in complex sugars and is located in the cell wall of the growing hyphae. Crf2 may be involved in the remodeling of the cell wall as well, and therefore in the growth and the virulence of the fungus.

Q4WI46

This is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Vial

Aspergillus fumigatus is a common airborne fungal pathogen for humans. It frequently causes an invasive aspergillosis (IA) in immunocompromised patients with poor prognosis. Potent antifungal drugs are very expensive and cause serious adverse effects. Their correct application requires an early and specific diagnosis of IA. Encoded by CRF1 gene, the Crf protein family contains three protein variants: Crf2 protein, amino acids (333 aa) long, Asp f9 protein (292 aa), first identified as an allergen using serum from Aspergillus-allergic patients and Crf1 (395 aa, also named Crh5). The glycosyol hydrolase/glycosidase Crf2 catalyzes the hydrolysis of glycosidic bonds in complex sugars and is located in the cell wall of the growing hyphae. Crf2 may be involved in the remodeling of the cell wall as well, and therefore in the growth and the virulence of the fungus.

>95% (SDS-PAGE)

Protein A purified from HEK293 cell culture supernatant.

Recognizes Crf2 from Aspergillus fumigatus. Possible cross-reactivity with Crf1, Crf2 and Asp f9 [Reference 2].

Non-hazardous

Primary Antibodies

12352203

Stable for at least 1 year after receipt when stored at -20°C.Stable for at least 1 week when stored at +4°C.