anti-LPS (Legionella pneumophila) mAb (rec.) (PHK121-H2)

Lipopolysaccharide; Endotoxin

1 mg/ml

32160000

liquid

Liquid. In PBS.

After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Please handle under sterile conditions to avoid contamination.

Legionella pneumophila (Strain Corby). Selection on whole cell.

Recombinant Antibody. Recognizes LPS from Legionella pneumophila. Applications: ELISA, WB. Clone: PHK121-H2. Isotype: Human IgG1. Formulation: Liquid. In PBS. Legionella are flagellated Gram-negative bacteria that belong to the class of gamma-proteobacteria. They are ubiquitously present in water habitats, including natural and man-made reservoirs where they infect and parasitize protozoa. In the host, a specialized compartment is formed, that enables replication of Legionella. When humans inhale aerosolized water contaminated with Legionella, alveolar macrophages can be infected, which may lead to a lifethreatening pneumonia called Legionnaires? disease. Due to the universal distribution of Legionella in water and their potential threat to human health, the Legionella concentration in water for human use must be strictly monitored, which is difficult since the standard detection still relies on lengthy cultivation and analysis of bacterial morphology. Specific and fast detection of L. pneumophila based on a highly stable antibody-based assay would be potentially useful.

This is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Vial

Legionella are flagellated Gram-negative bacteria that belong to the class of gamma-proteobacteria. They are ubiquitously present in water habitats, including natural and man-made reservoirs where they infect and parasitize protozoa. In the host, a specialized compartment is formed, that enables replication of Legionella. When humans inhale aerosolized water contaminated with Legionella, alveolar macrophages can be infected, which may lead to a lifethreatening pneumonia called Legionnaires? disease. Due to the universal distribution of Legionella in water and their potential threat to human health, the Legionella concentration in water for human use must be strictly monitored, which is difficult since the standard detection still relies on lengthy cultivation and analysis of bacterial morphology. Specific and fast detection of L. pneumophila based on a highly stable antibody-based assay would be potentially useful.

>95% (SDS-PAGE)

Protein A purified from HEK293 cell culture supernatant.

Recognizes LPS from Legionella pneumophila.

Non-hazardous

Primary Antibodies

12352203

Stable for at least 1 year after receipt when stored at -20°C.Stable for at least 1 week when stored at +4°C.