anti-Cdh2 (zebrafish) mAb (rec.) (SH1352-G9)

Neural Cadherin; N-cadherin

1 mg/ml

32160000

liquid

Liquid. In PBS.

After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Please handle under sterile conditions to avoid contamination.

Recombinant zebrafish Cdh2 domain 1 and domain 2 containing a C-terminal human Fc-tag.

Recombinant Antibody. Recognizes zebrafish Cdh2. Applications: ELISA, FACS, IHC, WB. Clone: SH1352-G9. Isotype: Human IgG1. Formulation: Liquid. In PBS. The cadherin superfamily of cell adhesion molecules regulate cell-cell interaction and are key factors in developmental morphogenesis and tissue repair. Cadherin-2 (N-cadherin; Cdh2), expressed in the vertebrate nervous system, is a type I transmembrane glycoprotein expressed in fusion to an N-terminal propeptide, necessary to avoid intracellular interaction of the molecule. Mature Cdh2 consists of five extracellular immunoglobulin (Ig)-like domains (Cdh2-ECDs), a single-pass transmembrane region and an intracellular domain for the interaction with the cell cytoskeleton, mainly mediated by beta-catenin binding. Throughout the early developmental stages of vertebrates, Cdh2 is expressed almost ubiquitously. Later stages its expression seems to be restricted to the central nervous system (CNS) in specific neuronal populations, but it also persists in cardiac muscle. In the central and peripheral nervous systems, Cdh2-dependent cell-cell interactions mediate a variety of neurodevelopmental processes including tissue architecture formation, neuronal migration, axon outgrowth, target recognition and synaptogenesis. Cdh2 levels are often increased during cancer invasion and metastatic behavior resulting in enhanced cell motility. For its role in tumor progression, Cdh2 has been considered a potential biomarker for the prediction of metastases and as an important prognostic factor. As a member of the cadherin superfamily of proteins, zebrafish Cdh2 (zCdh2) shares a high degree of amino acid homology with other members of this family. Zebrafish (Danio rerio) Cdh2-ECD has an overall amino acid identity of 63% to zebrafish Cadherin-4-ECD, with a peak of 68% in domains 1. The Ig-like structure of the 5 extracellular domains and the high sequence similarity to other cadherins requires a careful process of antibody generation against the zCdh2-ECD to avoid unwanted cross-reactivities and a thorough process of binding specificity characterization.

Q90275

This is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Vial

The cadherin superfamily of cell adhesion molecules regulate cell-cell interaction and are key factors in developmental morphogenesis and tissue repair. Cadherin-2 (N-cadherin; Cdh2), expressed in the vertebrate nervous system, is a type I transmembrane glycoprotein expressed in fusion to an N-terminal propeptide, necessary to avoid intracellular interaction of the molecule. Mature Cdh2 consists of five extracellular immunoglobulin (Ig)-like domains (Cdh2-ECDs), a single-pass transmembrane region and an intracellular domain for the interaction with the cell cytoskeleton, mainly mediated by beta-catenin binding. Throughout the early developmental stages of vertebrates, Cdh2 is expressed almost ubiquitously. Later stages its expression seems to be restricted to the central nervous system (CNS) in specific neuronal populations, but it also persists in cardiac muscle. In the central and peripheral nervous systems, Cdh2-dependent cell-cell interactions mediate a variety of neurodevelopmental processes including tissue architecture formation, neuronal migration, axon outgrowth, target recognition and synaptogenesis. Cdh2 levels are often increased during cancer invasion and metastatic behavior resulting in enhanced cell motility. For its role in tumor progression, Cdh2 has been considered a potential biomarker for the prediction of metastases and as an important prognostic factor. As a member of the cadherin superfamily of proteins, zebrafish Cdh2 (zCdh2) shares a high degree of amino acid homology with other members of this family. Zebrafish (Danio rerio) Cdh2-ECD has an overall amino acid identity of 63% to zebrafish Cadherin-4-ECD, with a peak of 68% in domains 1. The Ig-like structure of the 5 extracellular domains and the high sequence similarity to other cadherins requires a careful process of antibody generation against the zCdh2-ECD to avoid unwanted cross-reactivities and a thorough process of binding specificity characterization.

>95% (SDS-PAGE)

Protein A purified from HEK293 cell culture supernatant.

Recognizes zebrafish Cdh2.

Non-hazardous

Primary Antibodies

12352203

Stable for at least 1 year after receipt when stored at -20°C.Stable for at least 1 week when stored at +4°C.