BioStab PCR Optimizer
Code | Size | Price |
---|
AG-10T-0032-L001 | 1 ml | £66.00 |
Quantity:
AG-10T-0032-L005 | 5 ml | £172.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
AMBIENT
Storage:
+4°C
Documents
Further Information
Appearance:
Clear, light yellow liquid.
EClass:
32160000
Form (Short):
liquid
Formulation:
Filtered through a 0.22 µm filter. It is a 5X concentrated solution in sterile, bidestillated water and contains no proteins or preservatives.
Handling Advice:
Do not freeze.
Long Description:
PCR is a technique extensively used in different applications in molecular biology, molecular diagnostics and forensics. However, some sequences, for example the 5?-regions of many eukaryotic genes, are difficult to amplify. Incomplete separation of DNA strands results in little or no yield of desired product and multiple non-specific PCR-fragments. BioStab PCR Optimizer solves this problem and outperforms betaine. The mode of action of BioStab PCR optimizer comprises lowering the melting temperature of GC pairs without affecting that of AT pairs. The BioStab PCR optimizer is designed as a PCR additive which improves the yield and specificity of PCR reaction and allows amplification of difficult targets (e.g. sequences with >70% GC pairs). BioStab PCR optimizer enables the use of a wide range of annealing temperatures thereby eliminating the need for an optimization of amplification conditions. The BioStab PCR optimizer is provided as 5x concentrated solution. Working concentration in PCR mixture is 1x. For targets with >75% GC content a 2x solution is recommended.
Other data:
The BioStab PCR optimizer is designed as a PCR additive which improves the yield and specificity of PCR reaction and allows amplification of difficult targets (e.g. sequences with >70% GC pairs). BioStab PCR optimizer enables the use of a wide range of annealing temperatures thereby eliminating the need for an optimization of amplification conditions. The BioStab PCR optimizer is provided as 5x concentrated solution. Working concentration in PCR mixture is 1x. For targets with >75% GC content a 2x solution is recommended.
Package Type:
Vial
Product Description:
PCR is a technique extensively used in different applications in molecular biology, molecular diagnostics and forensics. However, some sequences, for example the 5?-regions of many eukaryotic genes, are difficult to amplify. Incomplete separation of DNA strands results in little or no yield of desired product and multiple non-specific PCR-fragments. BioStab PCR Optimizer solves this problem and outperforms betaine. The mode of action of BioStab PCR optimizer comprises lowering the melting temperature of GC pairs without affecting that of AT pairs. The BioStab PCR optimizer is designed as a PCR additive which improves the yield and specificity of PCR reaction and allows amplification of difficult targets (e.g. sequences with >70% GC pairs). BioStab PCR optimizer enables the use of a wide range of annealing temperatures thereby eliminating the need for an optimization of amplification conditions. The BioStab PCR optimizer is provided as 5x concentrated solution. Working concentration in PCR mixture is 1x. For targets with >75% GC content a 2x solution is recommended.
Transportation:
Non-hazardous
UNSPSC Category:
Biological Buffers
UNSPSC Number:
12161700
Use & Stability:
Stable for at least 2 years after receipt when stored at +4°C.