SARS-CoV-2 Spike P681H Antibody [9F7E4] (Alpha, Mu Variant)

Product Code: PSI-PM-9371

Supplier: ProSci

Code	Size	Price

PSI-PM-9371-0.02mg

0.02mg

£150.00

Quantity:

PSI-PM-9371-0.1mg

0.1mg

£515.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Isotype: IgG2a, Kappa

Antibody Clonality: Monoclonal

Antibody Clone: 9F7E4

Regulatory Status: RUO

Target Species: Virus

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Western Blot (WB)

Shipping:

blue ice or RT

Storage:

SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4

<strong>Figure 1 ELISA Validation of Alpha Variant Spike Antibodies with Spike S1 Protein of SARS-CoV-2 Variants </strong><br>
Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 μg/mL, incubate at 4 ˚C overnight.
Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, PM-9371, dilution: 200-1000 ng/mL, incubate at RT for 1 hr.
Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.
<strong>SARS-CoV-2 alpha variant spike antibody (PM-9371) can specifically detect alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants by ELISA. </strong>

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<strong>Figure 2 ELISA Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein </strong><br>
Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK), 1 μg/mL, incubate at 4 ˚C overnight.
Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, PM-9371, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr.
Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.
<strong>SARS-CoV-2 P681H Mutant Specific Spike antibody (PM-9371) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein (10-300) by ELISA. </strong>

3 / 4

<strong>Figure 3 WB Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein </strong><br>
Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK).
Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, PM-9371, 1 μg/mL, incubate at RT for 1 hr.
Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.
SARS-CoV-2 P681H Mutant Specific Spike antibody (PM-9371) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein (10-300) by WB. </strong>

4 / 4

<strong>Figure 4 ELISA Validation of P681H Mutant Specific Spike Antibodies with Mutant and WT Peptide </strong><br>
Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT (9091P) and alpha variant (B.1.1.7, UK) (9359P), 1 μg/mL, incubate at 4 ˚C overnight.
Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, PM-9371, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr.
Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.
<strong>SARS-CoV-2 P681H Mutant Specific Spike antibody (PM-9371) can specifically detect alpha variant spike S1 peptide (681H, 9359P), but not WT peptide (681P, 9091P). </strong>

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Documents

Further Information

Additional Names:

SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein

Application Note:

WB: 1 μg/mL.

Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody can specifically detect SARS-CoV-2 Alpha Variant (B.1.1.7, UK) S1 protein, but not SARS-CoV-2 WT Spike S1 protein by ELISA and WB. It can also detect mutant peptide (681H), but not WT peptide (681P). All other applications and species not yet tested.

Background:

In September of 2020 a new lineage of SARS-CoV-2, known as B.1.1.7 and named as Alpha variant, was discovered in the United Kingdom. This lineage was found to have developed 14 lineage-specific amino acid replacements and 3 deletions prior to its discovery. The transmission of alpha variant (B.1.1.7 lineage) was increased at least 50%. Increased severity and higher death rate were also found in apha variant. Alpha variant will not affect the effectiveness of COVID19 vaccine. One of the mutations associated with this lineage is a N501Y in the spike protein of the virus. It is believed that this mutation is able to increase the spike protein's affinity for the host ACE2 receptor and it has been associated with increased infectivity and virulence. B.1.1.7 viruses have also been shown to have a P681H mutation in the cleavage site of spike protein. This location is one of the residues that make up the furin cleavage site between S1 and S2 in spike protein.

Background References:

Duchene et al. Virus Evolution 6(2): veaa061.
Gu et al. Science 369(6511):1603-1607
Hoffmann et al. Molecular Cell 78(4):779-784.e5
Davies et al. Science 372(6538):eabg3055.
Davies et al. Nature. 593(7858):270-274.
Graham, et al. The Lancet Public Health. 6(5): e335-e345.
Horby et al. New & Emerging Threats Advisory Group. 2020;91:264-266.

Buffer:

SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (25%)

Immunogen:

Anti-SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike P681H antibody (PM-9371) was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 Alpha variant (B.1.1.7) Spike protein.

The immunogen is located within 650-700 amino acids of SARS-CoV-2 Alpha Variant (B.1.1.7, UK) Spike protein.

NCBI Gene ID #:

43740568

NCBI Official Name:

surface glycoprotein

NCBI Official Symbol:

NCBI Organism:

SARS-CoV-2

Physical State:

Liquid

Protein Accession #:

QHD43416

Protein GI Number:

1791269090

Purification:

SARS-CoV-2 (COVID-19) Alpha Variant (B.1.1.7, UK) Spike P681H Antibody is Protein A purified.

Research Area:

Infectious Disease,COVID-19

SPECIFICITY:

May cross-react with several virus of interest (VOI) variant lineages that contains P681H mutation, including B.11.318, B.1.621, B.1.621.1, P.3. But all of these lineages are rarely present in current pandemic.

Swissprot #:

P0DTC2

User NOte:

Optimal dilutions for each application to be determined by the researcher.