SARS-CoV-2 Spike RBD Neutralizing Antibody, monoclonal, Chimeric

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ProSci
Product Code: PSI-11-588
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-11-588-0.1mg0.1mg£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Antibody Isotype: Human IgG1/kappa
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
blue ice or RT
Storage:
For long term storage, the product should be stored at lyophilized state at -20˚C or lower..Please avoid repeated freeze-thaw cycles.This product is stable after storage at:.-20 to -70˚C for 12 months in lyophilized state from date of receipt;.-70˚C for 3 months under sterile conditions after reconstitution.

Images

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Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Detection SARS-CoV-2 S protein RBD by Sandwich ELISA Assay. Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 at 2 ug/mL (100 uL/well) can bind S protein RBD. And then add Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 at 1:5000. Detection was performed using HRP-conjugated streptavidin with sensitivity of 25 pg/mL (Routinely tested).
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Immobilized Human ACE2, His Tag at 2 ug/mL (100 uL/well) can bind pre-mixed increasing concentrations of Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 and 0.4 ug/mL (50 uL/well) Biotinylated S protein RBD, His,Avitag with a half maximal inhibitory concentration (IC50) of 0.33 ug/mL (Routinely tested).
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Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD, His Tag with an affinity constant of 0.375 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Detection SARS-CoV-2 S protein RBD by Sandwich ELISA Assay. Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 at 2 ug/mL (100 uL/well) can bind S protein RBD. And then add Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 at 1:5000. Detection was performed using HRP-conjugated streptavidin with sensitivity of 25 pg/mL (Routinely tested).
Immobilized Human ACE2, His Tag at 2 ug/mL (100 uL/well) can bind pre-mixed increasing concentrations of Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 and 0.4 ug/mL (50 uL/well) Biotinylated S protein RBD, His,Avitag with a half maximal inhibitory concentration (IC50) of 0.33 ug/mL (Routinely tested).
Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD, His Tag with an affinity constant of 0.375 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Documents

Further Information

Additional Names:
spike glycoprotein
Application Note:
This antibody can be paired with other Anti-SARS-CoV-2 Spike S1 antibodies to detect SARS-CoV-2 Spike S1 protein in sandwich ELISA or LFA assay.
Background:
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Buffer:
Lyophilized from 0.22 um filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified recombinant SARS-CoV-2 Spike S1 Protein. Pseudovirus assay shows that this antibody has potent neutralizing activity against pseudovirus bearing SARS-CoV-2 Spike protein.
NCBI Gene ID #:
43740568
NCBI Official Name:
Surface glycoprotein
NCBI Official Symbol:
S
NCBI Organism:
SARS-CoV-2
Physical State:
Lyophilized
Purification:
>95% as determined by SDS-PAGE.
Research Area:
Infectious Disease, COVID-19, Antibody Pairs
SPECIFICITY:
This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. No cross-reactivity is detected with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.
Swissprot #:
P0DTC2
User NOte:
Optimal dilutions for each application to be determined by the researcher.