ACE2 Antibody (HRP)

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ProSci
Product Code: PSI-3217-HRP
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3217-HRP-0.02mg0.02mg£150.00
Quantity:
PSI-3217-HRP-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
blue ice or RT
Storage:
ACE2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 ELISA Validation </strong><br> Coating Antigen: immunogen peptide, 3217P, 10 μg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, 3217-HRP or 3217, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. 3217 was detected by anti-rabbit HRP-conjugated secondary antibodies at 1:10,000, incubate at RT for 1 hr.
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<strong>Figure 1 ELISA Validation </strong><br> Coating Antigen: immunogen peptide, 3217P, 10 μg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, 3217-HRP or 3217, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. 3217 was detected by anti-rabbit HRP-conjugated secondary antibodies at 1:10,000, incubate at RT for 1 hr.

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Further Information

Additional Names:
ACE2 Antibody: ACEH, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, SARS-CoV receptor, SARS-CoV-2 receptor
Application Note:
IHC: 2 μg/mL
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested
Background:

ACE2 Antibody: Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. Homology modeling shows 2019-nCoV has a similar receptor-binding domain structure as SARS-CoV, which suggests COVID-19 (2019-nCoV) may use ACE2 as a receptor in humans for infection. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.

Background References:
  • Donoghue et al. Circ. Res. 2000;87:1-9.
  • Tipnis et al. J Biol. Chem. 2000;275:33238-43.
  • Li et al. Nature 2003;426:450-4.
  • Lu et al. The Lancet 2020 (published online).
  • Crackower et al. Nature 2002;417:822-8.
Buffer:
ACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
HRP
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (88%)
Immunogen:
Anti-ACE2 antibody (3217) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human ACE2.

The immunogen is located within the last 50 amino acids of ACE2.
ISOFORMS:
Human ACE2 has 2 isoforms, including isoform 1 (805aa, 93kD) and isoform 2 (555aa, 64kD). Mouse ACE2 also has 2 isoforms, including isoform 1 (805aa, 92kD) and isoform 2 (353aa, 40kD). Rat ACE2 has one isoform (805aa, 93kD). 3215 can detect human, mouse and rat.
NCBI Gene ID #:
59272
NCBI Official Name:
angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
NCBI Official Symbol:
ACE2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 93kD

Observed: 130 kD (7 N-linked glycosylation)
Protein Accession #:
NP_068576
Protein GI Number:
11225609
Purification:
ACE2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease
SPECIFICITY:
Anti-ACE2 has no cross response to ACE1.
Swissprot #:
Q9BYF1
User NOte:
Optimal dilutions for each application to be determined by the researcher.

References

  1. Wei, et al.Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection. Cell. 2020 Oct 20;S0092-8674(20)31392-1. doi: 10.1016/j.cell.2020.10.028.PMID: 33147444