CX3CR1 Antibody (biotin)

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ProSci
Product Code: PSI-2093-biotin
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-2093-biotin-0.02mg0.02mg£150.00
Quantity:
PSI-2093-biotin-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
blue ice or RT
Storage:
CX3CR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 ELISA Validation </strong><br> Coating Antigen: immunogen peptide, 2093P, 10 μg/mL, incubate at 4 ˚C overnight. Detection Antibodies: CX3CR1, 2093-biotin, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. 2093-biotin was detected by HRP-conjugated streptavidin at 1:5,000 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10,000 , incubate at RT for 1 hr.
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<strong>Figure 1 ELISA Validation </strong><br> Coating Antigen: immunogen peptide, 2093P, 10 μg/mL, incubate at 4 ˚C overnight. Detection Antibodies: CX3CR1, 2093-biotin, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. 2093-biotin was detected by HRP-conjugated streptavidin at 1:5,000 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10,000 , incubate at RT for 1 hr.

Documents

Further Information

Additional Names:
CX3CR1 Antibody: V28, CCRL1, GPR13, CMKDR1, GPRV28, CMKBRL1, CX3C chemokine receptor 1, Beta chemokine receptor-like 1, C-X3-C CKR-1
Background:
CX3CR1 Antibody: CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, murine, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1. Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration and adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.
Background References:
  • Raport et al. Gene 1995;163:295-9.
  • Combadiere et al. DNA Cell Biol 1995;14:673-80.
  • Combadiere et al. Biochem Biophys Res Commun 1998;253:728-32.
  • Harrison et al. Neurosci Lett 1994;169:85-9.
Buffer:
CX3CR1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
biotin
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (82%)
Immunogen:
Anti-CX3CR1 antibody (2093) was raised against a peptide corresponding to 20 amino acids near the amino terminus of mature human CX3CR1.

The immunogen is located within the first 50 amino acids of CX3CR1.
ISOFORMS:
Human CX3CR1 has 3 isoforms, including isoform 1 (355aa, 40kD), isoform 2 (387aa, 44kD), isoform 3 (362aa, 41kD). Mouse CX3CR1 has one isoform (354aa, 40kD) and rat CX3CR1 also has one isoform (354aa, 40kD). 2093 can detect all isoforms of human, mouse and rat.
NCBI Gene ID #:
1524
NCBI Official Name:
chemokine (C-X3-C motif) receptor 1
NCBI Official Symbol:
CX3CR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 40-44 kD

Observed: 50 kD
Protein Accession #:
NP_001328
Protein GI Number:
4503171
Purification:
CX3CR1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Chemokines & Cytokines,Cancer,Infectious Disease
Swissprot #:
P49238
User NOte:
Optimal dilutions for each application to be determined by the researcher.