TMEM41B (CT) Antibody

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ProSci
Product Code: PSI-9565
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9565-0.02mg0.02mg£150.00
Quantity:
PSI-9565-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
blue ice or RT
Storage:
TMEM41B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 Western Blot Validation in Mouse Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: TMEM41B 9565, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 2 Western Blot Validation in Rat Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TMEM41B 9565, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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<strong>Figure 3 Immunofluorescence Validation of TMEM41B in HeLa Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TMEM41B with 9565 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 4 Immunofluorescence Validation of TMEM41B in Human Lung</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung labeling TMEM41B with 9565 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 5 Immunofluorescence Validation of TMEM41B in Mouse Kidney </strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse kidney labeling TMEM41B with 9565 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 6 Immunofluorescence Validation of TMEM41B in Rat Lung</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling TMEM41B with 9565 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 1 Western Blot Validation in Mouse Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: TMEM41B 9565, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 Western Blot Validation in Rat Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TMEM41B 9565, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3 Immunofluorescence Validation of TMEM41B in HeLa Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TMEM41B with 9565 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 4 Immunofluorescence Validation of TMEM41B in Human Lung</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung labeling TMEM41B with 9565 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 5 Immunofluorescence Validation of TMEM41B in Mouse Kidney </strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse kidney labeling TMEM41B with 9565 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 6 Immunofluorescence Validation of TMEM41B in Rat Lung</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling TMEM41B with 9565 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Documents

Further Information

Additional Names:
TMEM41B Antibody: Transmembrane protein 41B, Protein stasimon, TMEM41B, KIAA0033.
Application Note:
WB: 2 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Background:
TMEM41B Antibody: TMEM41B is required for autophagosome formation and participates in early stages of autophagosome biogenesis at the ER membrane probably via mobilization of neutral lipids from lipid droplets. TMRM41B is a critical host factor required for infection by human coronaviruses SARS-CoV-2, HCoV-OC43, HCoV-NL63, and HCoV-229E, as well as all flaviviruses tested such as Zika virus and Yellow fever virus. It is required post-entry of the virus to facilitate the ER membrane remodeling necessary to form replication organelles.
Background References:
  • Moretti et al. EMBO Rep. 2018; 19(9):e45889.
  • Shoemaker et al. PLoS Biol. 2019; 17(4):e2007044.
  • Schneider et al. Cell 2021; 184(1):120-132.
  • Hoffmann et al. Cell 2021; 184(1):133-148.e20.
Buffer:
TMEM41B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine (100%); Chicken (100%); Chimpanzee (100%).
Immunogen:
Anti-TMEM41B antibody (9565) was raised against a peptide corresponding to 10 amino acids near the carboxyl terminus of human TMEM41B.

The immunogen is located within the last 50 amino acids of TMEM41B.
NCBI Gene ID #:
440026
NCBI Official Name:
transmembrane protein 41B
NCBI Official Symbol:
TMEM41B
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 33kD

Observed: 33 kD
Protein Accession #:
Q5BJD5
Protein GI Number:
74741383
Purification:
TMEM41B Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
Swissprot #:
Q5BJD5
User NOte:
Optimal dilutions for each application to be determined by the researcher.