TMPRSS2 (CT) Antibody

Contact us
ProSci
Product Code: PSI-9569
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9569-0.02mg0.02mg£150.00
Quantity:
PSI-9569-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
blue ice or RT
Storage:
TMPRSS2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 7
<strong>Figure 1 WB Validation in Human Testis </strong><br> Loading: 15 μg of lysate Antibodies: TMPRSS2 9569, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 1 μg/mL Lane 2: 2 μg/mL Lane 3: 4 μg/mL
2 / 7
<strong>Figure 2 Western Blot Validation in Mouse Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: TMPRSS2 9569, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
3 / 7
<strong>Figure 3 Western Blot Validation in Rat Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TMPRSS2 9569, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
4 / 7
<strong>Figure 4 Immunofluorescence Validation of TMPRSS2 in A549 Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling TMPRSS2 with 9569 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
5 / 7
<strong>Figure 5 Immunofluorescence Validation of TMPRSS2 in Human Lung</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung labeling TMPRSS2 with 9569 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
6 / 7
<strong>Figure 6 Immunofluorescence Validation of TMPRSS2 in Mouse Kidney</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse kidney labeling TMPRSS2 with 9569 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
7 / 7
<strong>Figure 7 Immunofluorescence Validation of TMPRSS2 in Rat Testis</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat Testis labeling TMPRSS2 with 9569 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
❮❯

<strong>Figure 1 WB Validation in Human Testis </strong><br> Loading: 15 μg of lysate Antibodies: TMPRSS2 9569, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 1 μg/mL Lane 2: 2 μg/mL Lane 3: 4 μg/mL
<strong>Figure 2 Western Blot Validation in Mouse Tissues </strong><br> Loading: 15 μg of lysates per lane. Antibodies: TMPRSS2 9569, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3 Western Blot Validation in Rat Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TMPRSS2 9569, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 4 Immunofluorescence Validation of TMPRSS2 in A549 Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling TMPRSS2 with 9569 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 5 Immunofluorescence Validation of TMPRSS2 in Human Lung</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung labeling TMPRSS2 with 9569 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 6 Immunofluorescence Validation of TMPRSS2 in Mouse Kidney</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse kidney labeling TMPRSS2 with 9569 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 7 Immunofluorescence Validation of TMPRSS2 in Rat Testis</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat Testis labeling TMPRSS2 with 9569 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Documents

Further Information

Additional Names:
TMPRSS2 Antibody: Transmembrane protease serine 2, Serine protease 10, PRSS10, Transmembrane protease serine 2 non-catalytic chain, Transmembrane protease serine 2 catalytic chain.
Application Note:
WB: 2 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Background:
TMPRSS2 Antibody: TMPRSS2 is a plasma membrane-anchored serine protease that participates in proteolytic cascades of relevance for the normal physiologic function of the prostate. Androgen-induced TMPRSS2 activates several substrates that include pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14 leading to extracellular matrix disruption and metastasis of prostate cancer cells. It facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via two independent mechanisms, proteolytic cleavage of ACE2 receptor which promotes viral uptake, and cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry. It proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). TMPRSS2 is essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9), and it is involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.
Background References:
  • Lucas et al. Cancer Discov. 2014; 4(11):1310-25.
  • Ko et al. Cancer Res. 2015; 75(14):2949-60.
  • Zang et al. Sci. Immunol. 2020; 5(47):eabc3582.
Buffer:
TMPRSS2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey (100%); Gorilla (100%); Cat (92%).
Immunogen:
Anti-TMPRSS2 antibody (9569) was raised against a peptide corresponding to 12 amino acids near the carboxyl terminus of human TMPRSS2.

The immunogen is located within the last 50 amino acids of TMPRSS2.
ISOFORMS:
Human TMPRSS2 has 2 isoforms, including isoform 1 (492aa, 54kD) and isoform 2 (529aa, 57.7kD). Mouse TMPRSS2 has 1 isoform (490aa, 54kD). Rat TMPRSS2 has one isoform (490aa, 53kD). 9569 can detect TMPRSS2 in human, mouse and rat.
NCBI Gene ID #:
7113
NCBI Official Name:
transmembrane serine protease 2
NCBI Official Symbol:
TMPRSS2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 54kD

Observed: 54 kD
Protein Accession #:
O15393
Protein GI Number:
115502469
Purification:
TMPRSS2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
Swissprot #:
O15393
User NOte:
Optimal dilutions for each application to be determined by the researcher.