SARS-CoV-2 (COVID-19) Membrane Antibody (biotin)

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ProSci
Product Code: PSI-9157-biotin
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9157-biotin-0.02mg0.02mg£150.00
Quantity:
PSI-9157-biotin-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Virus
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
blue ice or RT
Storage:
SARS-CoV-2 (COVID-19) Membrane antibody can be stored at 4 ˚ C for three months and -20 ˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 ELISA Validation </strong><br> Coating Antigen: immunogen peptide, 9157P, 10 μg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, 9157-biotin or 9157, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. 9157-biotin was detected by HRP-conjugated streptavidin at 1:5,000 and 9157 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10,000 , incubate at RT for 1 hr.
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<strong>Figure 2 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Membrane Protein in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) membrane antibody (9157-HRP, 2 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C, following by streptavidin-HRP conjugate at 20 μg/mL. Counter stained with Hematoxylin. SARS-COV-2 membrane protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
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<strong>Figure 1 ELISA Validation </strong><br> Coating Antigen: immunogen peptide, 9157P, 10 μg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, 9157-biotin or 9157, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. 9157-biotin was detected by HRP-conjugated streptavidin at 1:5,000 and 9157 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10,000 , incubate at RT for 1 hr.
<strong>Figure 2 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Membrane Protein in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) membrane antibody (9157-HRP, 2 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C, following by streptavidin-HRP conjugate at 20 μg/mL. Counter stained with Hematoxylin. SARS-COV-2 membrane protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.

Documents

Further Information

Additional Names:
SARS-CoV-2/SARS-CoV Matrix Antibody: SARS-CoV-2 Matrix protein, SARS Matrix protein, Membrane protein, E1 glycoprotein, M protein
Application Note:
IHC: 2 μg/mL
Antibody validated: Immunohistochemistry in COVID-19 patient samples. All other applications and species not yet tested.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019?20 coronavirus outbreak (1). SARS-CoV-2 is the seventh member of the enveloped, positive-stranded RNA viruses that are able to infect humans. The SARS-CoV-2 genome, like other coronaviruses, encodes for multiple structural and nonstructural proteins. The structural proteins include spike protein (S), envelope protein (E), membrane glycoprotein (M), nucleocapsid phosphoprotein (N), and the nonstructural proteins include open reading frame 1ab (ORF1ab), ORF3a, ORF6, ORF7a, ORF8, and ORF10 (2).The membrane (M) protein or matrix protein is the most abundant structural protein and defines the shape of the viral envelope (3). It is an integral membrane protein involved in the budding of the viral particles and interacts with S (Spike) protein. It involves in organization of the nucleoprotein inside, which includes many copies of the N (nucleocapsid) protein bound to the genomic RNA. The M protein holds dominant cellular immunogenicity and has been determined as a protective antigen in humoral responses, which suggests it would serve as a potential target in vaccine design (4).
Background References:
  • Zhu et al. N Engl J Med. 2020 Feb 20;382(8):727-733.
  • Kiyotani et al. J Hum Genet. 2020 Jul;65(7):569-575
  • Neuman et al. J Struct Biol. 2011;174(1):11?22.
  • Liu et al. J Infect Dis. 2010;202(8):1171-80.
Buffer:
SARS-CoV-2 (COVID-19) Membrane Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
biotin
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV Matrix protein: (100%)
Immunogen:
Anti-SARS-CoV-2 (COVID-19) Membrane antibody (biotin) (9157) was raised against a peptide corresponding to 13 amino acids near the center of SARS-CoV-2 (COVID-19) Membrane protein.

The immunogen is located between 130-180 amino acids of the SARS-CoV-2 (COVID-19) Membrane protein.
NCBI Gene ID #:
43740571
NCBI Official Name:
Membrane protein
NCBI Official Symbol:
M
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
Protein Accession #:
QJW69073
Protein GI Number:
1800455127
Purification:
SARS-CoV-2 (COVID-19) Membrane Antibody is affinity chromatography
Research Area:
Infectious Disease,COVID-19
Swissprot #:
P0DTC5
User NOte:
Optimal dilutions for each application to be determined by the researcher.