SARS-CoV-2 (COVID-19) ORF3b Antibody

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ProSci
Product Code: PSI-9281
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9281-0.02mg0.02mg£150.00
Quantity:
PSI-9281-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
blue ice or RT
Storage:
SARS-CoV-2 (COVID-19) ORF3b antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) ORF3b Protein </strong><br> Antibodies: SARS-CoV-2 (COVID-19) ORF3b Antibody, 9281. A direct ELISA was performed using SARS-CoV-2 ORF3b recombinant protein (10-005) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF3b antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL
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<strong>Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF3b Protein</strong><br> Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF3b recombinant protein (10-005). Antibodies: SARS-CoV-2 (COVID-19) ORF3b, 9281, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 μg/mL and Lane 2: 0.5 μg/mL
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<strong>Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) ORF3b Protein </strong><br> Antibodies: SARS-CoV-2 (COVID-19) ORF3b Antibody, 9281. A direct ELISA was performed using SARS-CoV-2 ORF3b recombinant protein (10-005) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF3b antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL
<strong>Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF3b Protein</strong><br> Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF3b recombinant protein (10-005). Antibodies: SARS-CoV-2 (COVID-19) ORF3b, 9281, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 μg/mL and Lane 2: 0.5 μg/mL

Documents

Further Information

Additional Names:
ORF3b protein, Acessary protein 3b, ns3b, Non-structural protein 3b, ORF3b
Application Note:
WB: 0.25 μg/mL

Antibody validated: SARS-CoV-2 (COVID-19) ORF3b antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 ORF3b recombinant protein by ELISA and WB. All other applications and species not yet tested.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF3b, ORF9b, ORF9c and ORF10. ORF3b may play a role in interferon antiviral system evasion (3).
Background References:
  • Gorbalenya. bioRxiv: 2020.
  • Hui et al. Int J Infect Dis. 2020;91:264-266.
  • Konno et al. Cell Rep. 2020; 32:108185.
Buffer:
SARS-CoV-2 (COVID-19) ORF3b antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV ORF3b: (92%).
Immunogen:
Anti-SARS-CoV-2 (COVID-19) ORF3b antibody (9281) was raised against a peptide corresponding to 13 amino acids near the amino-terminus of SARS-CoV-2 (COVID-19) ORF3b protein.

The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) ORF3b protein.
NCBI Gene ID #:
43740569
NCBI Official Name:
ORF3a protein
NCBI Official Symbol:
ORF3a
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
17kD
Protein Accession #:
P0DTC3
Protein GI Number:
1835921851
Purification:
SARS-CoV-2 (COVID-19) ORF3b Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
Swissprot #:
P0DTC6
User NOte:
Optimal dilutions for each application to be determined by the researcher.