SARS-CoV-2 (COVID-19) ORF8 Antibody

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ProSci
Product Code: PSI-9289
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9289-0.02mg0.02mg£150.00
Quantity:
PSI-9289-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
blue ice or RT
Storage:
SARS-CoV-2 (COVID-19) ORF8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF8 Protein</strong><br> Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF8 recombinant protein (10-436). Antibodies: SARS-CoV-2 (COVID-19) ORF8, 9289, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 μg/mL and Lane 2: 2 μg/mL
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<strong>Figure 2 ELISA Validation </strong><br> Antibodies: SARS-CoV-2 (COVID-19) ORF8 Antibody, 9289. A direct ELISA was performed using SARS-CoV-2 ORF8 immunogen peptide (9289P)) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF8 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL
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<strong>Figure 1 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF8 Protein</strong><br> Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF8 recombinant protein (10-436). Antibodies: SARS-CoV-2 (COVID-19) ORF8, 9289, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 μg/mL and Lane 2: 2 μg/mL
<strong>Figure 2 ELISA Validation </strong><br> Antibodies: SARS-CoV-2 (COVID-19) ORF8 Antibody, 9289. A direct ELISA was performed using SARS-CoV-2 ORF8 immunogen peptide (9289P)) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF8 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL

Documents

Further Information

Additional Names:
ORF8 protein, ns8, ORF8, Non-structural protein 8
Application Note:
WB: 1 μg/mL

Antibody validated: SARS-CoV-2 (COVID-19) ORF8 antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 ORF8 recombinant protein by WB. All other applications and species not yet tested.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF8, ORF9b, ORF9c and ORF10. ORF8 may play a role in modulating host immune response (Probable). May play a role in blocking host IL17 cytokine by its interaction with host IL17RA (3).
Background References:
  • Gorbalenya. bioRxiv: 2020.
  • Hui et al. Int J Infect Dis. 2020;91:264-266.
  • Chan et al. Lancet. 2020; 395:514-523.
Buffer:
SARS-CoV-2 (COVID-19) ORF8 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti-SARS-CoV-2 (COVID-19) ORF8 antibody (9289) was raised against a peptide corresponding to 13 amino acids near the central domain of SARS-CoV-2 (COVID-19) ORF8 protein.

The immunogen is located within 30-80 amino acids of the SARS-CoV-2 (COVID-19) ORF8 protein.
NCBI Gene ID #:
43740577
NCBI Official Name:
ORF8 protein
NCBI Official Symbol:
ORF8
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
14kD
Protein Accession #:
P0DTC8
Protein GI Number:
1835921949
Purification:
SARS-CoV-2 (COVID-19) ORF8 Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
SPECIFICITY:
ORF8 Antibody is predicted to not cross-react with other coronavirus family members.
Swissprot #:
P0DTC8
User NOte:
Optimal dilutions for each application to be determined by the researcher.