SARS-CoV-2 (COVID-19) ORF9c Antibody

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ProSci
Product Code: PSI-9291
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-9291-0.02mg0.02mg£150.00
Quantity:
PSI-9291-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
blue ice or RT
Storage:
SARS-CoV-2 (COVID-19) ORF9c antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF9c Overexpressed 293 Cells</strong><br> Loading: 10 μg per lane of 293 cell lysate from WT and SARS-CoV-2 (COVID-19) ORF9c transfected cells. Antibodies: SARS-CoV-2 (COVID-19) ORF9c 9291, 1 μg/ml, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF9c Overexpressed 293 Cells</strong><br> Loading: 10 μg per lane of 293 cell lysate from WT and SARS-CoV-2 (COVID-19) ORF9c transfected cells. Antibodies: SARS-CoV-2 (COVID-19) ORF9c, 9291, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 μg/mL and Lane 2: 0.5 μg/mL
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<strong>Figure 3 ELISA Validation </strong><br> Antibodies: SARS-CoV-2 (COVID-19) ORF9c Antibody, 9291. A direct ELISA was performed using SARS-CoV-2 ORF9c immunogen peptide (9291P)) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF9c antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL
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<strong>Figure 1 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF9c Overexpressed 293 Cells</strong><br> Loading: 10 μg per lane of 293 cell lysate from WT and SARS-CoV-2 (COVID-19) ORF9c transfected cells. Antibodies: SARS-CoV-2 (COVID-19) ORF9c 9291, 1 μg/ml, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF9c Overexpressed 293 Cells</strong><br> Loading: 10 μg per lane of 293 cell lysate from WT and SARS-CoV-2 (COVID-19) ORF9c transfected cells. Antibodies: SARS-CoV-2 (COVID-19) ORF9c, 9291, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 μg/mL and Lane 2: 0.5 μg/mL
<strong>Figure 3 ELISA Validation </strong><br> Antibodies: SARS-CoV-2 (COVID-19) ORF9c Antibody, 9291. A direct ELISA was performed using SARS-CoV-2 ORF9c immunogen peptide (9291P)) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF9c antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL

Documents

Further Information

Additional Names:
ORF9c protein, Uncharacterized protein 14, ORF14, ORF9c
Application Note:
WB: 0.25-1 μg/mL

Antibody validated: SARS-CoV-2 (COVID-19) ORF9c antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 ORF9c in transfected human cells by WB. All other applications and species not yet tested
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF8, ORF9b, ORF9c and ORF10. ORF9c may play a role in host-virus interaction.
Background References:
  • Gorbalenya. bioRxiv: 2020.
  • Hui et al. Int J Infect Dis. 2020;91:264-266.
Buffer:
SARS-CoV-2 (COVID-19) ORF9c antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti-SARS-CoV-2 (COVID-19) ORF9c antibody (9291) was raised against a peptide corresponding to 16 amino acids near the amino-terminus of SARS-CoV-2 (COVID-19) ORF9c protein.

The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) ORF9c protein.
NCBI Official Symbol:
ORF9c
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
8kD
Protein Accession #:
P0DTD3
Protein GI Number:
1835922084
Purification:
SARS-CoV-2 (COVID-19) ORF9c Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
SPECIFICITY:
ORF9c Antibody is predicted to not cross-react with other coronavirus family members.
Swissprot #:
P0DTD3
User NOte:
Optimal dilutions for each application to be determined by the researcher.