PDL1 Detection Set
| Code | Size | Price |
|---|
| PSI-SD8500-1Set | 1Set | £313.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Llama
Antibody Isotype: sdab
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Flow Cytometry
- Immunocytochemistry (ICC)
- Immunofluorescence (IF)
Shipping:
dry ice
Storage:
PD-L1 antibodies should be stored in working aliquots at -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Background:
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts.
Background References:
- Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
- Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
- LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.
Buffer:
PBS containing 0.02% sodium azide.
Concentration:
Antibody 1 mg/mL
Conjugate:
Unconjugated
Immunogen:
PD-L1 antibodies were raised against a recombinant protein corresponding to amino acids 19 - 239 of human PD-L1.
Physical State:
Liquid
Purification:
PD-L1 Antibodies are affinity chromatography purified via Nickel column.Antibodies are supplied as a His-tagged purified protein.??They also contains a myc-tag for detection.
Set Contents:
20 μg:
PD-L1 Antibody [F6A9], Cat. No. SD8639
PD-L1 Antibody [F2G2], Cat. No. SD8641
100 μg:
cMyc-tag Antibody [5G5H7], Cat. No. PM-7669
PD-L1 Antibody [F6A9], Cat. No. SD8639
PD-L1 Antibody [F2G2], Cat. No. SD8641
100 μg:
cMyc-tag Antibody [5G5H7], Cat. No. PM-7669
User Note:
Optimal dilutions for each application to be determined by the researcher. Antibodies have a C-terminal HIS 6-Tag followed by a myc-Tag for detection and have been tested using ProSci's PM-7669 (cMyc-tag Antibody [5G5H7]).
References
- Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
- Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
- LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.