SARS-CoV-2 (COVID-19) S1 RBD Detection Set

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ProSci
Product Code: PSI-SD9400
Supplier: ProSci
CodeSizePrice
PSI-SD9400-1Set1Set£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Llama
Antibody Isotype: sdab
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Virus
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
Shipping:
dry ice
Storage:
Antibodies should be stored in working aliquots at -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Documents

Further Information

Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a Spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Coronavirus invades cells through Spike (S) glycoproteins, a class I fusion protein. It is the major viral surface protein that coronavirus uses to bind to the human cell surface receptor. It also mediates the fusion of host and viral cell membrane, allowing the virus to enter human cells and begin infection (3). The spike protein is the major target for neutralizing antibodies and vaccine development (4). The protein modeling suggests that there is strong interaction between Spike protein receptor-binding domain and its host receptor angiotensin-converting enzyme 2 (ACE2), which regulate both the cross-species and human-to-human transmissions of COVID-19 (5). The recent study has shown that the SARS-CoV-2 spike protein binds ACE2 with higher affinity than SARS-CoV spike protein (6).
Background References:
  • Gorbalenya. bioRxiv: 2020.
  • Hui et al. Int J Infect Dis. 2020;91:264-266.
  • Belouzard et al. Viruses. 2012;4(6):1011-33.
  • Lee et al. J Virol. 2006;80(8):4079-87.
Buffer:
PBS containing 0.02% sodium azide.
Concentration:
Antibody 1 mg/mL
Conjugate:
Unconjugated
Immunogen:
SARS-CoV-2 S protein RBD containing C-terminal His Tag. The protein was expressed in human 293 cells (HEK293). It contains amino acids Arg 319 - Lys 537.
Physical State:
Liquid
Purification:
Affinity chromatography purified via Nickel column. Antibodies are supplied as a His-tagged purified protein.??They also contains a myc-tag for detection.
Set Contents:
20 μg:
SARS-CoV-2 (COVID-19) S1 RBD Antibody [RBD-2B9], Cat. No. SD9437
SARS-CoV-2 (COVID-19) S1 RBD Antibody [T5P4-A12], Cat. No. SD9439
SARS-CoV-2 (COVID-19) S1 RBD Antibody [T5P7-G10], Cat. No. SD9441
SARS-CoV-2 (COVID-19) S1 RBD Antibody [T4P5-H12], Cat. No. SD9507
SARS-CoV-2 (COVID-19) S1 RBD Antibody [T3P1-C8], Cat. No. SD9511

100 μg:
cMyc-tag Antibody [5G5H7], Cat. No. PM-7669
User Note:
Optimal dilutions for each application to be determined by the researcher.

References

  1. Gorbalenya. bioRxiv: 2020.
  2. Hui et al. Int J Infect Dis. 2020;91:264-266.
  3. Belouzard et al. Viruses. 2012;4(6):1011-33.
  4. Lee et al. J Virol. 2006;80(8):4079-87.