SARS-CoV-2 (COVID-19) Envelope Antibody
| Code | Size | Price |
|---|
| PSI-20-274-0.1mg | 0.1mg | £758.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Sheep
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Virus
Application: Western Blot (WB)
Shipping:
dry ice
Storage:
Aliquot and store at -20˚C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
SARS-CoV-2-E Protein, SARS-CoV-2-Envelope Protein
Application Note:
Detection Antibodies: 1 μg/ml Ab, 5% BSA in TBS-T, 4˚C overnight
Blocking: 5% milk in TBS-T, 1h room temp
Secondary Antibodies: 1:15,000 donkey anti-sheep, 1:15,000 donkey anti-mouse, in TBS-T, 1h room temp
Wash: TBS-T, 4x 10 min
Blocking: 5% milk in TBS-T, 1h room temp
Secondary Antibodies: 1:15,000 donkey anti-sheep, 1:15,000 donkey anti-mouse, in TBS-T, 1h room temp
Wash: TBS-T, 4x 10 min
Background:
Coronavirus envelope (E) proteins are short (1 residues) polypeptides that contain at least one transmembrane (TM) domain and a cluster of 2-3 juxtamembrane cysteines. These proteins are involved in viral morphogenesis and tropism, and their absence leads in some cases to aberrant virions, or to viral attenuation. In common to other viroporins, coronavirus envelope proteins increase membrane permeability to ions, plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis. Activates the host NLRP3 inflammasome, leading to IL-1beta overproduction.
Buffer:
Antibody supplied in PBS.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
GST-SARS-CoV2 E Protein
Molecular Weight:
53 kDa
NCBI Gene ID #:
43740570
NCBI Official Name:
Envelope small membrane protein
NCBI Official Symbol:
E
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
Purification:
Antibodies are affinity purified against the antigen and eluted with low pH.
Research Area:
Infectious Disease,COVID-19
Swissprot #:
P0DTC4
User NOte:
Optimal dilutions for each application to be determined by the researcher.