Secreted IL-2 Receptor alpha Recombinant Protein
| Code | Size | Price |
|---|
| PSI-40-622-0.005mg | 0.005mg | £300.00 |
Quantity:
| PSI-40-622-0.025mg | 0.025mg | £428.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Hamster
Regulatory Status: RUO
Target Species: Human
Shipping:
blue ice or RT
Storage:
The lyophilized Secreted IL-2 Receptor alpha recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Secreted IL-2 Receptor alpha is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Secreted IL-2 Receptor alpha recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Documents
Further Information
Additional Names:
CD25, IL2R, TCGFR, IDDM10, Interleukin-2 receptor subunit alpha, TAC antigen, IL-2 receptor subunit alpha
Background:
The IL-2 receptor system consists of three non-covalently linked subunits termed IL-2R alpha, IL-2R beta, and IL-2R gamma.??The IL-2R alpha is a type I transmembrane protein consisting of a 219 amino acid extracellular domain, a 19 amino acid transmembrane domain and a 13 amino acid intracellular domain, which is not involved in the transduction of IL-2 signals.??Proteolytic processing of IL-2R alpha releases the entire extracellular domain of IL-2R alpha, thereby generating a 219 amino acid soluble protein called soluble IL-2R alpha (sIL-2R alpha).??The homodimeric form binds IL-2 (KD=10mM) and facilitates IL-2 signaling.??The secreted sIL-2R alpha is expressed on leukemia cells, lymphoma cells, and newly activated T and B cells, as well as on approximately 10% of NK cells.??Recombinant Human sIL-2 Receptor alpha is a 24.8 kDa protein containing 219 amino acid residues consisting of only the extracellular domain of IL-2R alpha. As a result of glycosylation, Recombinant Human sIL-2 Receptor alpha migrates with an apparent molecular mass of approximately 40-50 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.
Biological Activity:
Determined by its ability to increase the proliferation effect of IL-2 in murine CTLL-2 cells. In the presence of 1 ng/ml of recombinant IL-2, the expected ED50 for this effect is between 0.5 - 1.5 ug/ml.
NCBI Gene ID #:
3559
NCBI Official Name:
interleukin 2 receptor, alpha
NCBI Official Symbol:
IL2RA
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
NP_000408.1
Protein GI Number:
4557667
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Recombinant Protein Sequence:
#N/A
Source:
CHO cells
Swissprot #:
P01589