SARS-CoV-2 (COVID-19) Spike (D614G) (Stable Trimer) Recombinant Protein
| Code | Size | Price |
|---|
| PSI-21-814-50ug | 50ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Target Species: Human
Shipping:
blue ice or RT
Storage:
Short Term StorageL: +4°C, Long Term Storage: -20°C, Handling Advice: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. For maximum product recovery after thawing, centrifuge the vial before opening the cap. Use/Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
Further Information
Additional Names:
2019-nCoV Spike Protein S
Background:
SARS-CoV-2 shares 79.5% sequence identity with SARS-CoV and is 96.2% identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. The original Wuhan strain of the virus has become quickly replaced by its more transmissible variant, mainly determined by a single amino acid point mutation D614G.
The coronaviral genome encodes four major structural proteins: the Spike (S) protein, Nucleocapsid (N) protein, Membrane/Matrix (M) protein and the Envelope (E) protein. The SARS Envelope (E) protein contains a short palindromic transmembrane helical hairpin that seems to deform lipid bilayers, which may explain its role in viral budding and virion envelope morphogenesis. The SARS Membrane/Matrix (M) protein is one of the major structural viral proteins. It is an integral membrane protein involved in the budding of the viral particles and interacts with SARS Spike (S) protein and the Nucleocapsid (N) protein. The N protein contains two domains, both of them bind the virus RNA genome via different mechanisms.
The CoV Spike (S) protein assembles as trimer and plays the most important role in viral attachment, fusion and entry. It is composed of a short intracellular tail, a transmembrane anchor and a large ectodomain that consists of a receptor binding S1 subunit (RBD domain) and a membrane-fusing S2 subunit. The S1 subunit contains a receptor binding domain (RBD), which binds to the cell surface receptor angiotensin-converting enzyme 2 (ACE2) present at the surface of epithelial cells. It has been demonstrated that certain mutations and the inclusion of trimerization motif can stabilize recombinant Spike protein trimers.
The recombinant protein SARS-CoV-2 Spike Protein (D614G) (Stable Trimer) (rec.) (His) could be useful for structural biology research, vaccine development, serological diagnostic kit development or neutralizing antibody screening.
Biological Activity:
Binds to the human SARS-CoV-2 receptor ACE2. Binds to anti-SARS-CoV-2 Spike antibodies in serum or plasma.
Buffer:
Lyophilized. Contains PBS and 0.5% trehalose.
Concentration:
0.2mg/ml after reconstitution.
NCBI Gene ID #:
43740568
NCBI Official Name:
surface glycoprotein
NCBI Official Symbol:
S
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Lyophilized
Predicted Molecular Weight:
~190kDa (SDS-PAGE)
Protein Accession #:
P0DTC2
Purity:
>90% (SDS-PAGE)
Recombinant Protein Sequence:
#N/A
Source:
HEK293 cells
Swissprot #:
P0DTC2