Siglec-1 / CD169 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-11-194-0.1mg | 0.1mg | £729.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Shipping:
blue ice or RT
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
Siglec-1,CD169,SIGLEC1,SN
Application Note:
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 175.1 kDa. The protein migrates as 200-220 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Background:
Alternate names for sialoadhesin include siglec-1 and CD169 (cluster of differentiation 169). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. It is defined as an I-type lectin, since it contains 17 immunoglobulin (Ig) domains (one variable domain and 16 constant domains), and thus also belongs to the immunoglobulin superfamily (IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid. Binds to SPN/CD43 on T-cells.
Background References:
- A Hartnell, et al. Blood. 2001. 97:288-96.
- S Kelm, et al. Curr Biol. 1994. 4(11):965-72.
- Nathan R Zaccai, et al. J Mol Biol. 2007 . 365(5):1469-79.
Buffer:
MES,NaCl,pH7.5
Fusion Tag:
His Tag
Physical State:
Lyophilized
Predicted Molecular Weight:
175.1 kDa
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
#N/A
Source:
HEK293 cells